PMID: 8586624May 1, 1995Paper

Changes of proteasomes and cathepsins activities and their expression during differentiation of C2C12 myoblasts

Journal of Biochemistry
C EbisuiM Monden

Abstract

C2C12 myoblasts fuse to form multinucleated myotubes and express muscle specific proteins during differentiation. To elucidate developmental regulation of intracellular proteolytic systems, enzymatic activities, protein and mRNA levels of proteasomes (20S and 26S) and lysosomal cathepsins (B, L, and H) were examined. Myoblasts were differentiated fully to myotubes 6 days after starting differentiation. In this developmental process, the 26S proteasome activity decreased, while the 20S proteasome activity increased. Expression of proteasome subunits of 20S (RC2, RC8) and regulatory components of 26S (S4, S7) was down-regulated, though total protein levels of proteasomes showed no remarkable changes. On the other hand, enzymatic activities of cathepsins B and B + L increased in association with an increase of their transcriptional and translational levels. Expression of their specific endogenous inhibitor, cystatin beta, also increased. Maturation of the lysosomal proteolytic system was tightly linked to the differentiation process. These results suggested that signals for differentiation of myoblasts mediate a change of intracellular proteolytic systems, involving up-regulation of lysosomal cathepsins and down-regulation of prot...Continue Reading

Citations

Oct 30, 2013·Molecular and Cellular Biology·Ziyou CuiAldrin V Gomes
Oct 3, 2019·Scientific Reports·Antonio F Millan-CubilloJosefina Blasco
Jun 7, 2003·American Journal of Physiology. Endocrinology and Metabolism·Stéphanie DuguezDamien Freyssenet
Dec 22, 2006·American Journal of Physiology. Cell Physiology·Marianne VermaelenOlivier Cazorla
Aug 3, 2018·Biological & Pharmaceutical Bulletin·Hiroshi Sakane, Kenji Akasaki
May 29, 2014·The Biochemical Journal·Elliott M McMillan, Joe Quadrilatero
Jul 27, 2002·Adverse Drug Reactions and Toxicological Reviews·David Mantle, Victor R Preedy
Aug 8, 1998·The International Journal of Biochemistry & Cell Biology·Y UedaN E Forsberg
Jul 24, 2002·Experimental Hematology·Cheng Yao ChenStephen T Koury
Mar 14, 2003·Biochimica Et Biophysica Acta·Reinout P HesselinkGer J Van der Vusse
Aug 30, 2005·The International Journal of Biochemistry & Cell Biology·Daniel BechetDidier Attaix
Jun 16, 2015·Journal of Proteome Research·Marie-Catherine Le BihanIrina Kratchmarova

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