Changes of pyridoxal kinase expression and activity in the gerbil hippocampus following transient forebrain ischemia

Neuroscience
I K HwangMoo Ho Won

Abstract

In the previous study, we observed chronological alterations of glutamic acid decarboxylase (GAD), which is the enzyme converting glutamate into GABA. GAD isoforms (GAD65 and GAD67) differ substantially in their interactions with cofactor pyridoxal 5'-phosphate, which is catalyzed by pyridoxal kinase (PLK). In the present study, we examined the chronological changes of PLK expression and activity in the hippocampus after 5 min transient forebrain ischemia in gerbils. PLK immunoreactivity in the sham-operated group was detected weakly in the hippocampus. Ischemia-related change of PLK immunoreactivity in the hippocampus was significant in the hippocampal cornu ammonis (CA1)region, not in the hippocampal CA2/3 region and dentate gyrus. PLK immunoreactivity was observed in non-pyramidal GABAergic neurons at 30 min to 3 h after ischemic insult. At 12 h after ischemic insult, PLK immunoreactivity was shown in many CA1 pyramidal cells as well as some non-pyramidal cells. At this time point, PLK immunoreactivity and protein content was highest after ischemia. Thereafter, PLK immunoreactivity and protein content is decreased time-dependently by 4 days after ischemic insult. Four days after ischemia, some astrocytes expressed PLK in the...Continue Reading

References

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Citations

Jun 4, 2008·Biological & Pharmaceutical Bulletin·Chun Sung KimDo Kyung Kim
Oct 22, 2010·The Journal of Veterinary Medical Science·Choong Hyun LeeMoo-Ho Won
Mar 11, 2010·Journal of Clinical Biochemistry and Nutrition·Kumi KimuraAsako Takenaka
Feb 8, 2007·Experimental Animals·Ki-Yeon YooMoo Ho Won
Dec 1, 2015·Neurochemistry International·Antero SalminenMikko Hiltunen

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