Changes of salivary biomarkers under different storage conditions: effects of temperature and length of storage

Biochemia Medica
Tomas BarrancoDamián Escribano

Abstract

In this report, we aimed to examine the stability of various analytes in saliva under different storage conditions. Alpha-amylase (AMY), cholinesterase (CHE), lipase (Lip), total esterase (TEA), creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LD), lactate (Lact), adenosine deaminase (ADA), Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability (FRAS), cupric reducing antioxidant capacity (CUPRAC), uric acid (UA), catalase (CAT), advanced oxidation protein products (AOPP) and hydrogen peroxide (H2O2) were colorimetrically measured in saliva obtained by passive drool from 12 healthy voluntary donors at baseline and after 3, 6, 24, 72 hours, 7 and 14 days at room temperature (RT) and 4 ºC, and after 14 days, 1, 3 and 6 months at - 20 ºC and - 80 ºC. At RT, changes appeared at 6 hours for TEA and H2O2; 24 hours for Lip, CK, ADA and CUPRAC; and 72 hours for LD, Lact, FRAS, UA and AOPP. At 4 ºC changes were observed after 6 hours for TEA and H2O2; 24 hours for Lip and CUPRAC; 72 hours for CK; and 7 days for LD, FRAS and UA. At - 20 ºC changes appeared after 14 days for AST, Lip, CK and LD; and 3 months for TEA and H2O2. At - 80 ºC observed changes were after 3 months for TEA and H2O2. In...Continue Reading

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Citations

May 21, 2020·Journal of Clinical Medicine·Jose J CeronAsta Tvarijonaviciute
Sep 3, 2020·International Journal of Environmental Research and Public Health·José María Giménez-EgidoJosé Joaquín Cerón
Jun 13, 2020·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Pía López-JornetTvarijonaviciute Asta
Nov 15, 2020·Research in Veterinary Science·Isabel BarrancoXiomara Lucas

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