Changes of serum-induced ornithine decarboxylase activity and putrescine content during aging of IMR-90 human diploid fibroblasts

Journal of Cellular Physiology
K Y ChenA Y Liu

Abstract

The roles of ornithine decarboxylase (ODC, EC 4.1.1.17) and polyamines in cellular aging were investigated by examining serum-induced changes of these parameters in quiescent IMR-90 human diploid fibroblasts as a function of their population doubling level (PDL) and in human progeria fibroblasts. Serum stimulation caused increases of ODC and DNA synthesis in IMR-90 human diploid fibroblasts, with maximal values occurring, respectively, 10 hr and 22 hr after serum stimulation. Both serum-induced ODC activity and DNA synthesis in IMR-90 cells were found to be inversely related to their PDL. Maximal ODC activity and DNA synthesis in young cells (PDL = approximately 18-22) were, respectively, five-fold and six-fold greater than that in old cells (PDL = approximately 50-55), which in turn were comparable or slightly higher than that in progeria fibroblasts. Polyamine contents (putrescine, spermidine, and spermine) in quiescent IMR-90 cells did not show significant PDL-dependency. The putrescine and spermine contents in quiescent progeria cells were comparable to those in quiescent IMR-90 cells. The spermidine content in quiescent progeria cells, however, was extremely low, less than half of that in quiescent IMR-90 cells. Serum stim...Continue Reading

References

Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D H Russell, P J Stambrook
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·K Y Chen, E S Canellakis
Jul 1, 1979·The Journal of Investigative Dermatology·S Goldstein
Jul 1, 1979·The Journal of Investigative Dermatology·L Hayflick
Jan 1, 1976·Annual Review of Biochemistry·C W Tabor, H Tabor
Sep 1, 1977·Experimental Cell Research·P E Duffy, L T Kremzner
Jan 26, 1976·Biochemical and Biophysical Research Communications·K ChenE S Canellakis
Jan 1, 1975·Experimental Cell Research·O HebyR Baserga
Jan 1, 1974·Methods in Enzymology·J S Nolan, L Packer
Jul 1, 1973·Analytical Biochemistry·E Ross, G Schatz
Jan 1, 1984·Annual Review of Biochemistry·C W Tabor, H Tabor
Aug 1, 1984·Mechanisms of Ageing and Development·G Scalabrino, M E Ferioli
Nov 1, 1982·The American Journal of Physiology·A E Pegg, P P McCann
Dec 1, 1961·Experimental Cell Research·L HAYFLICK, P S MOORHEAD
Dec 1, 1964·Acta Physiologica Scandinavica·J JAENNEM SIIMES
Mar 1, 1965·Experimental Cell Research·L HAYFLICK

❮ Previous
Next ❯

Citations

Jul 1, 1990·Mechanisms of Ageing and Development·H Shigeoka, H C Yang
Nov 15, 2003·Journal of Anti-aging Medicine·Michael Fossel
Feb 13, 1987·Biochemical and Biophysical Research Communications·K Y Chen, Z F Chang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.