Changes on the viral capsid surface during the evolution of porcine circovirus type 2 (PCV2) from 2009 till 2018 may lead to a better receptor binding

Virus Evolution
Ruifang WeiHans J Nauwynck

Abstract

Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus-associated diseases (PCVAD). Three major PCV2 genotypes (PCV2a, PCV2b, and PCV2d) have been identified globally. Despite their worldwide distribution, the prevalence and genetic evolution of PCV2 in Belgium has not previously been determined. In this study, 319 samples from animals suffering from diseases likely to be associated with PCV2 were collected from 2009 to 2018 and analysed by virus titration. The overall prevalence of PCV2 in PCVAD-suspected cases was 15.7 per cent (50/319). The phylogenetic analysis demonstrated that at least three genotypes (PCV2a, PCV2b, and PCV2d) circulated in Belgium from 2009 till 2018, and that PCV2 evolved from PCV2a to PCV2b and from PCV2d-1 to PCV2d-2. Sequence comparison among the forty-three PCV2 isolates showed that they had 89.7-100 per cent nucleotide-sequence and 88.5-100 per cent amino-acid-sequence identities. Three amino acid sites were under positive selection. Three-dimensional analysis of genotype-specific amino acids revealed that most of the mutations were on the outside of the cap protein with a few conserved mutations present on the inner side. Mutations toward more basic amino acids were ...Continue Reading

References

Apr 4, 1998·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·G M AllanB M Adair
Jul 31, 1998·Veterinary Pathology·M KiupelD M Haines
Aug 22, 2000·The Journal of General Virology·P NawagitgulP S Paul
Oct 6, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·I W WalkerG M Allan
Nov 22, 2000·The Veterinary Quarterly·G G LabarqueM B Pensaert
Sep 15, 2001·Veterinary Microbiology·R E SanchezM B Pensaert
Oct 29, 2002·The Veterinary Quarterly·J Segalés, M Domingo
Apr 5, 2003·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·G M Rodríguez-ArriojaM Domingo
May 22, 2004·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Romeo E SanchezMaurice B Pensaert
Feb 17, 2005·Bioinformatics·Sergei L Kosakovsky Pond, Simon D W Frost
Jun 28, 2005·Nucleic Acids Research·Dina Schneidman-DuhovnyHaim J Wolfson
Nov 14, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Tanja OpriessnigPatrick G Halbur
Jan 25, 2008·BMC Bioinformatics·Yang Zhang
Jul 1, 2008·The Veterinary Record·J SegalésG Allan
Aug 19, 2008·Applied and Environmental Microbiology·Kyoung-Ho KimJin-Woo Bae
Dec 17, 2008·Veterinary Microbiology·Danja D WiederkehrXaver Sidler
Apr 15, 2009·Archives of Virology·David J LefebvreH J Nauwynck
Sep 24, 2009·Veterinary Microbiology·Daniel VerreaultCaroline Duchaine
Dec 15, 2010·Veterinary Microbiology·R BluntK Mellits
Mar 25, 2011·Clinical and Vaccine Immunology : CVI·Benjamin R TribleRaymond R R Rowland
Oct 25, 2011·BMC Veterinary Research·Dipongkor SahaHans J Nauwynck
Apr 12, 2012·The Journal of General Virology·Dipongkor SahaHans J Nauwynck
Jul 19, 2012·PLoS Genetics·Ben MurrellSergei L Kosakovsky Pond

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
EditSeq

Software Mentioned

Clustal
PyMOL
MegAlign
SYMMDOCK
TASSER
MEGA
molecular evolutionary genetics analysis ( MEGA )
- TASSER )
FUBAR
SLAC

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.