Changing a conserved amino acid in R2R3-MYB transcription repressors results in cytoplasmic accumulation and abolishes their repressive activity in Arabidopsis

The Plant Journal : for Cell and Molecular Biology
Meiliang ZhouYanmin Wu

Abstract

Sub-group 4 R2R3-type MYB transcription factors, including MYB3, MYB4, MYB7 and MYB32, act as repressors in phenylpropanoid metabolism. These proteins contain the conserved MYB domain and the ethylene-responsive element binding factor-associated amphiphilic repression (EAR) repression domain. Additionally, MYB4, MYB7 and MYB32 possess a putative zinc-finger domain and a conserved GY/FDFLGL motif in their C-termini. The protein 'sensitive to ABA and drought 2' (SAD2) recognizes the nuclear pore complex, which then transports the SAD2-MYB4 complex into the nucleus. Here, we show that the conserved GY/FDFLGL motif contributes to the interaction between MYB factors and SAD2. The Asp → Asn mutation in the GY/FDFLGL motif abolishes the interaction between MYB transcription factors and SAD2, and therefore they cannot be transported into the nucleus and cannot repress their target genes. We found that MYB4(D261N) loses the capacity to repress expression of the cinnamate 4-hydroxylase (C4H) gene and biosynthesis of sinapoyl malate. Our results indicate conservation among MYB transcription factors in terms of their interaction with SAD2. Therefore, the Asp → Asn mutation may be used to engineer transcription factors.

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Jul 24, 1987·Nucleic Acids Research·R TöpferH H Steinbiss
Dec 5, 1998·The Plant Journal : for Cell and Molecular Biology·H D KranzB Weisshaar
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Sep 14, 2004·The Plant Journal : for Cell and Molecular Biology·Ilona M ZimmermannJoachim F Uhrig
Oct 8, 2004·The Plant Journal : for Cell and Molecular Biology·Keren Bracha-DroriNir Ohad
Dec 9, 2004·The Plant Journal : for Cell and Molecular Biology·Jeremy PrestonRoger W Parish
Aug 8, 2006·The Plant Journal : for Cell and Molecular Biology·Paul E VersluesJian-Kang Zhu
May 5, 2007·Nucleic Acids Research·Sitao Wu, Yang Zhang
Nov 27, 2007·The Plant Journal : for Cell and Molecular Biology·Antonio GonzalezAlan M Lloyd
Aug 3, 2010·Trends in Plant Science·Christian DubosLoïc Lepiniec
May 19, 2011·Applied Microbiology and Biotechnology·Mei-Liang ZhouYi-Xiong Tang
Dec 10, 2013·Plant & Cell Physiology·Silvia FornaléDavid Caparros-Ruiz
Mar 26, 2015·Applied Microbiology and Biotechnology·Meiliang ZhouYanmin Wu

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Citations

Feb 3, 2018·Journal of Experimental Botany·Kaixuan ZhangMeiliang Zhou
May 13, 2018·The Plant Journal : for Cell and Molecular Biology·Jinbo LiMeiliang Zhou
Jan 24, 2020·The Plant Journal : for Cell and Molecular Biology·Chinmayee PandaXu Li
Aug 11, 2019·Nucleic Acids Research·Peter S MillardMeike Burow
Apr 28, 2017·Frontiers in Plant Science·Yu YangHuiming Zhang
Apr 10, 2019·Plant Biotechnology Journal·Jinbo LiMeiliang Zhou
Aug 3, 2019·Trends in Plant Science·Marc BehrMarie Baucher
Apr 4, 2021·International Journal of Molecular Sciences·Huiling YanXiyang Zhao
Jul 3, 2021·International Journal of Molecular Sciences·Xiaopei WangYuan Zheng

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