Changing muscle function with sustained glial derived neurotrophic factor treatment of rabbit extraocular muscle

PloS One
Krysta FitzpatrickLinda K McLoon

Abstract

Recent microarray and RNAseq experiments provided evidence that glial derived neurotrophic factor (GDNF) levels were decreased in extraocular muscles from human strabismic subjects compared to age-matched controls. We assessed the effect of sustained GDNF treatment of the superior rectus muscles of rabbits on their physiological and morphological characteristics, and these were compared to naïve control muscles. Superior rectus muscles of rabbits were implanted with a sustained release pellet of GDNF to deliver 2μg/day, with the contralateral side receiving a placebo pellet. After one month, the muscles were assessed using in vitro physiological methods. The muscles were examined histologically for alteration in fiber size, myosin expression patterns, neuromuscular junction size, and stem cell numbers and compared to age-matched naïve control muscles. GDNF resulted in decreased force generation, which was also seen on the untreated contralateral superior rectus muscles. Muscle relaxation times were increased in the GDNF treated muscles. Myofiber mean cross-sectional areas were increased after the GDNF treatment, but there was a compensatory increase in expression of developmental, neonatal, and slow tonic myosin heavy chain iso...Continue Reading

References

Jan 1, 1990·European Journal of Applied Physiology and Occupational Physiology·J F PattonE A Harman
Jan 10, 1982·The Journal of Comparative Neurology·A Hughes, D I Vaney
Jul 1, 1994·Journal of Applied Physiology·B D JohnsonG C Sieck
Apr 1, 1994·The American Journal of Physiology·A G Nelson, W J Thompson
Aug 28, 1998·Cytokine & Growth Factor Reviews·M Sendtner
Mar 1, 2002·Muscle & Nerve·Linda K McLoon, Jonathan D Wirtschafter
Aug 27, 2003·Investigative Ophthalmology & Visual Science·Linda K McLoon, Stephen P Christiansen
Oct 11, 2003·Molecular and Cellular Neurosciences·Jennifer ChenChristopher S von Bartheld
Feb 26, 2004·Muscle & Nerve·Carlos B MantillaGary C Sieck
Sep 29, 2004·Investigative Ophthalmology & Visual Science·Jennifer Chen, Christopher S von Bartheld
Sep 17, 2005·The Journal of Physiology·Eve-Lyne BélairRichard Robitaille
Oct 31, 2006·Journal of AAPOS : the Official Publication of the American Association for Pediatric Ophthalmology and Strabismus·Linda K McLoonStephen P Christiansen
Dec 17, 2009·Investigative Ophthalmology & Visual Science·Tian LiChristopher S von Bartheld
Jun 12, 2010·Investigative Ophthalmology & Visual Science·Stephen P ChristiansenLinda K McLoon
Feb 1, 2011·Experimental Cell Research·Kristen M KallestadLinda K McLoon
Feb 1, 2011·Pflügers Archiv : European journal of physiology·Tian LiChristopher S von Bartheld
Jul 13, 2012·Investigative Ophthalmology & Visual Science·Amy L AltickChristopher S von Bartheld
Apr 2, 2013·Neuropharmacology·Agnes Thalhammer, Lorenzo A Cingolani
Jun 2, 2015·Investigative Ophthalmology & Visual Science·Christy L WilloughbyLinda K McLoon
Jun 2, 2015·Investigative Ophthalmology & Visual Science·Christy L WilloughbyLinda K McLoon
Jul 17, 2015·Skeletal Muscle·Stefano SchiaffinoCarlo Reggiani
Sep 20, 2015·Journal of Molecular Neuroscience : MN·Micaela Johanna GlatDaniel Offen
Dec 17, 2015·Skeletal Muscle·Bradley PawlikowskiBradley B Olwin
Oct 22, 2016·Investigative Ophthalmology & Visual Science·Andrea B AgarwalChristopher S von Bartheld
Nov 2, 2016·Investigative Ophthalmology & Visual Science·Krysta R NelsonLinda K McLoon
Nov 8, 2016·Investigative Ophthalmology & Visual Science·Linda K McLoonMichael J Mustari
Nov 8, 2016·Investigative Ophthalmology & Visual Science·Mythri PullelaVallabh E Das
Nov 8, 2017·Current Ophthalmology Reports·Mayank VermaLinda K McLoon
Jan 19, 2018·Investigative Ophthalmology & Visual Science·Linda K McLoonFatima Pedrosa Domellöf

❮ Previous
Next ❯

Citations

Jul 23, 2021·Investigative Ophthalmology & Visual Science·Jolene C Rudell, Linda K McLoon

❮ Previous
Next ❯

Methods Mentioned

BETA
RNAseq
transgenic
force measurements

Software Mentioned

Bioquant Image Analysis System
GraphPad Prism
Bioquant

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.