Apr 14, 2020

SETD2 is an actin lysine methyltransferase

BioRxiv : the Preprint Server for Biology
R. N. H. SeervaiCheryl Lyn Walker

Abstract

SET-domain-containing-2 (SETD2) was identified as the methyltransferase responsible for the histone 3 lysine 36 trimethyl (H3K36me3) mark of the histone code. Most recently, SETD2 has been shown to be a dual-function remodeler that regulates genome stability via methylation of dynamic microtubules during mitosis and cytokinesis. Here we show that actin is a bona fide target for methylation by SETD2 in vitro and in cells. Antibodies against the SETD2 trimethyl lysine epitope recognize methylated actin, with this methyl mark localizing to areas of active actin cytoskeleton reorganization in migrating cells. Disruption of this methylation activity causes defects in actin polymerization and impairs collective cell migration. Together, these data identify SETD2 as a multifunctional cytoskeletal remodeler regulating methylation and polymerization of actin filaments, and provide new avenues for understanding how defects in SETD2 drive disease via aberrant cytoskeletal methylation.

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Mentioned in this Paper

Metabolic Process, Cellular
Study
In Vivo
Genes
Circadian Clocks
HMGN2P11
LHY protein, Arabidopsis
Mutant Proteins
Laboratory
Plants, Transgenic

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