PMID: 9160336Jan 1, 1997Paper

Channel activity caused by a Bacillus thuringiensis delta-endotoxin preparation depends on the method of activation

Molecular Membrane Biology
D P SmedleyD J Ellar

Abstract

The spontaneous insertion of Bacillus thuringiensis Cry delta-endotoxins into planar lipid bilayers to form discrete channels in the absence of receptors is the subject of conflicting reports in the literature. Because these proteins are synthesized as protoxins requiring proteolytic activation for conversion to the active form, differences in the in-vitro protocol used for this activation could be responsible for the contradictory results. To investigate this, CrylA(c) toxin was activated by different procedures, and its ability to release glucose entrapped within liposomes and to form channels in planar lipid bilayers assessed. The toxin preparations exhibited widely differing activities on the lipid membranes; SDS-PAGE and immunoblot analysis suggested that variations in the protein profile of the activated samples could be responsible. These findings raise important practical considerations for further in-vitro studies into the mechanism of action of these toxins.

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Citations

Mar 21, 2002·Biochimica Et Biophysica Acta·Luke MassonRoland Brousseau
Oct 19, 2011·The Journal of Biological Chemistry·Nicolas GroulxRikard Blunck
Dec 19, 2002·Biochimica Et Biophysica Acta·Olivier PeyronnetJean-Louis Schwartz
Nov 24, 2001·Biochimica Et Biophysica Acta·G GuihardJ L Schwartz
Aug 31, 1999·Archives of Insect Biochemistry and Physiology·B Oppert
Feb 1, 2011·FEMS Microbiology Letters·Shuyuan GuoJie Zhang

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