PMID: 2125473Oct 2, 1990Paper

Channeling of TCA cycle intermediates in cultured Saccharomyces cerevisiae

Biochemistry
B SümegiC R Malloy

Abstract

Oxidation of [3-13C]propionate was studied in cultured yeast cells, and the distribution of label in the 2- and 3-positions of alanine was detected by 13C NMR. [3-13C]Propionate forms [2-13C]succinyl-CoA in the mitochondria which then enters the citric acid cycle and forms malate through two symmetrical intermediates, succinate and fumarate. If these symmetrical intermediates randomly diffuse from one enzyme to the next in mitochondria as is normally assumed, then 13C labeling in malate C2 and C3 must be equal. However, any direct transfer of metabolites from site to site between succinate thiokinase, succinate dehydrogenase, and fumarase would result in an uneven distribution of 13C in malate C2 and C3 and any molecules derived from malate. Since pyruvate may be derived from malate via the malic enzyme and subsequently converted into alanine by transamination, any 13C asymmetry in alanine C2 and C3 must directly reflect the 13C distribution in the malate pool. During oxidation of [3-13C]propionate, we detect a significant quantity of labeled alanine, where 13C enrichment in C3 is significantly higher than that in C2. Inhibition of succinate dehydrogenase with malonate or creating conditions that increase the chances of a back-...Continue Reading

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Citations

May 19, 2004·The International Journal of Biochemistry & Cell Biology·W J MalaisseA Sener
Sep 22, 2009·Chemical Reviews·Scott Severance, Iqbal Hamza
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B S Negrutskii, M P Deutscher
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B A ScalettarC R Hackenbrock
May 1, 1991·Trends in Biochemical Sciences·R G Shulman
May 1, 1991·Trends in Biochemical Sciences·R Rognstad
Oct 1, 1991·Biochemical Medicine and Metabolic Biology·B M Woodfin, L E Davis
Jun 16, 2007·Archives of Biochemistry and Biophysics·Eric R GraybillJohn W Hawes
Aug 1, 1997·Biochemical and Molecular Medicine·I VerbruggenW J Malaisse
Dec 1, 1996·Cell Biochemistry and Function·A D Sherry, C R Malloy
May 28, 2016·Microbial Cell Factories·Nils J H AvereschJens O Krömer
Dec 1, 1996·Cell Biochemistry and Function·J Ovádi, P A Srere
May 17, 2017·Nature Communications·Youjun ZhangToshihiro Obata
Sep 22, 1992·Biochemistry·B SumegiP A Srere
Sep 7, 1991·Journal of Theoretical Biology·R G Shulman
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Sep 25, 2019·Research : a Science Partner Journal·Alisdair R FernieLee J Sweetlove
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Dec 16, 2020·The Journal of Chemical Physics·Clare F MegarityFraser A Armstrong
May 21, 2003·The Journal of Experimental Biology·Raul K Suarez

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