PMID: 3143403Sep 6, 1988Paper

Channels at the catalytic site of glycogen phosphorylase b: binding and kinetic studies with the beta-glycosidase inhibitor D-gluconohydroximo-1,5-lactone N-phenylurethane

D BarfordL N Johnson


Regions of low packing density in the vicinity of the catalytic site of glycogen phosphorylase b are described with the aid of a computer program that generates a contour map in which the contour level is inversely proportional to the packing density in the protein. It is shown that, although there is no direct route from the catalytic site to the surface, there are two possible channels that could allow access for substrates following conformational changes in the enzyme. The first channel, channel 1, leads from the catalytic site to the surface close to the nucleoside inhibitor site and requires movements of residues 280-285 and Arg 569 in order to obtain access. Previous crystallographic experiments have shown that in the presence of substrates or R-state inhibitors these parts of the polypeptide chain undergo large conformational changes. The properties of the second channel (channel 2), which is the more extensive channel, have been investigated with the potent beta-glycosidase inhibitor D-gluconohydroximo-1,5-lactone N-phenylurethane (PUG). Crystallographic binding studies at 2.4-A resolution show that the compound binds neatly at the catalytic site of phosphorylase b. The glucopyranosylidene ring, in the half-chair confo...Continue Reading


Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·K Feldmann, W E Hull
Jul 5, 1979·Journal of Molecular Biology·S Sprang, R J Fletterick
Jan 1, 1987·CRC Critical Reviews in Biochemistry·A J Kirby
May 2, 1988·European Journal of Biochemistry·N G OikonomakosL N Johnson
Jun 17, 1986·Biochemistry·A A RashinB Honig
Jan 13, 1987·Biochemistry·H FrauenfelderM L Connolly
Jul 1, 1970·Canadian Journal of Biochemistry·H D EngersN B Madsen
Dec 25, 1974·Journal of Molecular Biology·L N JohnsonK S Wilson
Sep 15, 1974·Journal of Molecular Biology·L O FordR Tjian
Aug 16, 1969·Nature·R Wolfenden
Feb 14, 1971·Journal of Molecular Biology·B Lee, F M Richards
Apr 1, 1983·Biochemical Society Transactions·L N JohnsonY S Babu
Jan 1, 1981·The International Journal of Biochemistry·V Dombrádi

❮ Previous
Next ❯


Sep 1, 1992·Protein Science : a Publication of the Protein Society·D D LeonidasT G Sotiroudis
Dec 1, 1995·Protein Science : a Publication of the Protein Society·N G OikonomakosK R Acharya
Feb 5, 1990·Journal of Molecular Biology·L N JohnsonP J McLaughlin
Sep 1, 1992·Journal of Molecular Graphics·J S Delaney
May 1, 1997·Nature Structural Biology·M O'ReillyL N Johnson
May 2, 1988·European Journal of Biochemistry·N G OikonomakosL N Johnson
Nov 25, 2004·Journal of Molecular Biology·Alison L Cuff, Andrew C R Martin
Oct 11, 2005·Bioorganic & Medicinal Chemistry·Melissa PerreiraJohn A Hanover
Aug 1, 1989·Archives of Biochemistry and Biophysics·A C PapageorgiouD D Leonidas
Jul 5, 1989·Journal of Molecular Biology·K R AcharyaD C Phillips

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.