PMID: 8986757Dec 24, 1996Paper

Chaperone activity and structure of monomeric polypeptide binding domains of GroEL

Proceedings of the National Academy of Sciences of the United States of America
R ZahnA R Fersht

Abstract

The chaperonin GroEL is a large complex composed of 14 identical 57-kDa subunits that requires ATP and GroES for some of its activities. We find that a monomeric polypeptide corresponding to residues 191 to 345 has the activity of the tetradecamer both in facilitating the refolding of rhodanese and cyclophilin A in the absence of ATP and in catalyzing the unfolding of native barnase. Its crystal structure, solved at 2.5 A resolution, shows a well-ordered domain with the same fold as in intact GroEL. We have thus isolated the active site of the complex allosteric molecular chaperone, which functions as a "minichaperone." This has mechanistic implications: the presence of a central cavity in the GroEL complex is not essential for those representative activities in vitro, and neither are the allosteric properties. The function of the allosteric behavior on the binding of GroES and ATP must be to regulate the affinity of the protein for its various substrates in vivo, where the cavity may also be required for special functions.

References

Mar 1, 1992·Protein Science : a Publication of the Protein Society·P V ViitanenG H Lorimer
Nov 4, 1991·FEBS Letters·T E Gray, A R Fersht
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jan 1, 1966·Advances in Protein Chemistry·A Hvidt, S O Nielsen
Sep 1, 1995·The Journal of Biological Chemistry·Z W WhiteE Eisenstein
Aug 20, 1993·Journal of Molecular Biology·T E Gray, A R Fersht
Feb 1, 1996·Nature Structural Biology·D C BoisvertP B Sigler
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R J Ellis, F U Hartl
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·F J Corrales, A R Fersht
Nov 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt, M E Murphy
Mar 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·G J Kleywegt, T A Jones

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Citations

Jun 22, 2005·Journal of Molecular Biology·George StanD Thirumalai
Feb 27, 2003·Biochemical and Biophysical Research Communications·Danielle Amatore, François Baneyx
Sep 23, 2003·Journal of Molecular Biology·Arthur F MonzingoJon D Robertus
Jan 8, 1999·Biochimica Et Biophysica Acta·N S PrestonM G Gore
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·E Quaite-Randall, A Joachimiak
Mar 31, 1998·Current Opinion in Structural Biology·A Horovitz
Jul 11, 1998·The Biochemical Journal·N A RansonH R Saibil
Jan 1, 1997·Folding & Design·J E CoyleS E Radford
Sep 7, 2013·Journal of the American Chemical Society·Sam Tonddast-Navaei, George Stan
Sep 26, 1997·Nature Structural Biology·H E WhiteH R Saibil
Nov 5, 1999·Protein Science : a Publication of the Protein Society·Q WangA R Fersht
Jul 1, 2009·International Journal of Molecular Sciences·Victor V Marchenkov, Gennady V Semisotnov
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·A P Ben-ZviP Goloubinoff
Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·G M Clore, A M Gronenborn
Aug 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·A I JewettJ-E Shea
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·J Ma, M Karplus
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·J CarmichaelD C Rubinsztein
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·J ChatellierA R Fersht
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·R ZahnK Wüthrich
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·A M BuckleA R Fersht
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·M M AltamiranoA R Fersht
Nov 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Naoki TanakaShigeru Kunugi
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·J D WangJ S Weissman
Sep 21, 2001·Journal of Molecular Biology·T A FukamiK Miki
Oct 24, 2009·Cellular and Molecular Life Sciences : CMLS·Andrew I Jewett, Joan-Emma Shea
Nov 28, 2015·Protein Engineering, Design & Selection : PEDS·Olga A SharapovaAlexey N Fedorov
Aug 12, 2009·Biochemical and Biophysical Research Communications·Ching-Yi LinKuang-Wen Liao
Nov 26, 2008·Progress in Biophysics and Molecular Biology·Tapan K ChaudhuriAditi Maheshwari
Aug 30, 2008·Bio Systems·E PechkovaC Nicolini
Nov 13, 2004·Protein Science : a Publication of the Protein Society·Naoki TanakaShigeru Kunugi
Aug 7, 2008·Journal of Peptide Science : an Official Publication of the European Peptide Society·Abolfazl BarzegarNader Sheibani
Sep 12, 2012·Biotechnology Journal·Satoshi YamaguchiTeruyuki Nagamune
Sep 22, 2006·Journal of Molecular Biology·A I Jewett, J-E Shea

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