Chaperone activity of recombinant maize chloroplast protein synthesis elongation factor, EF-Tu

European Journal of Biochemistry
Damodara RaoZoran Ristic

Abstract

The protein synthesis elongation factor, EF-Tu, is a protein that carries aminoacyl-tRNA to the A-site of the ribosome during the elongation phase of protein synthesis. In maize (Zea mays L) this protein has been implicated in heat tolerance, and it has been hypothesized that EF-Tu confers heat tolerance by acting as a molecular chaperone and protecting heat-labile proteins from thermal aggregation and inactivation. In this study we investigated the effect of the recombinant precursor of maize EF-Tu (pre-EF-Tu) on thermal aggregation and inactivation of the heat-labile proteins, citrate synthase and malate dehydrogenase. The recombinant pre-EF-Tu was purified from Escherichia coli expressing this protein, and mass spectrometry confirmed that the isolated protein was indeed maize EF-Tu. The purified protein was capable of binding GDP (indicative of protein activity) and was stable at 45 degrees C, the highest temperature used in this study to test this protein for possible chaperone activity. Importantly, the recombinant maize pre-EF-Tu displayed chaperone activity. It protected citrate synthase and malate dehydrogenase from thermal aggregation and inactivation. To our knowledge, this is the first observation of chaperone activi...Continue Reading

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Citations

Jul 21, 2014·Enzyme and Microbial Technology·Keum-Young AhnJeewon Lee
Jul 25, 2009·Plant Physiology and Biochemistry : PPB·Joon-Yung ChaDaeyoung Son
Sep 4, 2007·Journal of Plant Physiology·Zoran RisticBenjamin P DeRidder
May 15, 2007·Journal of Plant Physiology·Zoran RisticP V Vara Prasad
Apr 2, 2016·BioMed Research International·Aayudh DasJai S Rohila
Oct 4, 2011·Biochemical and Biophysical Research Communications·Madhuri SuraganiNasreen Z Ehtesham
Oct 7, 2005·Molecular & Cellular Proteomics : MCP·Jean-Benoit PeltierKlaas J van Wijk
Jun 14, 2018·The Journal of Biological Chemistry·Anne WilkeningWolfgang Voos
Aug 28, 2007·Proteomics·Dong-Gi LeeByung-Hyun Lee
Mar 1, 2009·Functional Plant Biology : FPB·Urška BukovnikZoran Ristic
Nov 30, 2006·The Journal of Biological Chemistry·Hiroaki SuzukiNono Takeuchi

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