Chaperone properties of bacterial elongation factor EF-Tu.

The Journal of Biological Chemistry
T CaldasG Richarme

Abstract

Elongation factor Tu (EF-Tu) is involved in the binding and transport of the appropriate codon-specified aminoacyl-tRNA to the aminoacyl site of the ribosome. We report herewith that the Escherichia coli EF-Tu interacts with unfolded and denatured proteins as do molecular chaperones that are involved in protein folding and protein renaturation after stress. EF-Tu promotes the functional folding of citrate synthase and alpha-glucosidase after urea denaturation. It prevents the aggregation of citrate synthase under heat shock conditions, and it forms stable complexes with several unfolded proteins such as reduced carboxymethyl alpha-lactalbumin and unfolded bovine pancreatic trypsin inhibitor. The EF-Tu.GDP complex is much more active than EF-Tu.GTP in stimulating protein renaturation. These chaperone-like functions of EF-Tu occur at concentrations that are at least 20-fold lower than the cellular concentration of this factor. These results suggest that EF-Tu, in addition to its function in translation elongation, might be implicated in protein folding and protection from stress.

References

May 6, 1976·Nature·G R Jacobson, J P Rosenbusch
Mar 1, 1992·Molecular Microbiology·A WeijlandA Parmeggiani
Jul 9, 1992·Nature·H WiechUrsula Jakob
May 1, 1991·Journal of Bacteriology·C C Young, R W Bernlohr
Jan 1, 1990·Methods in Enzymology·M Cull, C S McHenry
Aug 1, 1989·Trends in Biochemical Sciences·R J Ellis, S M Hemmingsen
Jul 4, 1986·Cell·J E Rothman, S L Schmid
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H WolfA Parmeggiani
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·T BlumenthalK Weber
Mar 1, 1970·Archives of Biochemistry and Biophysics·H WeissbachJ Hachmann
Nov 21, 1970·Nature·A A TraversR F Schleif
Sep 8, 1965·Biochemical and Biophysical Research Communications·J A Hathaway, D E Atkinson
Oct 14, 1994·Science·N ShiinaE Nishida
Jun 1, 1994·Trends in Biochemical Sciences·M Sprinzl
Jan 1, 1993·Annual Review of Biochemistry·J P Hendrick, F U Hartl
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J P HendrickMartin Wiedmann
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·T HesterkampB Bukau
Jan 1, 1996·Biochimie·S Mottagui-Tabar, L A Isaksson
Jun 20, 1997·The Journal of Biological Chemistry·G Richarme, T Caldas
Jan 24, 1998·The Journal of Biological Chemistry·W KudlickiB Hardesty

Citations

May 23, 2001·Archives of Biochemistry and Biophysics·G KramerB Hardesty
Dec 10, 1999·Folia Microbiologica·B KraalC Kleanthous
Aug 23, 2011·Applied Microbiology and Biotechnology·Andoni Ramirez-GarciaFernando Luis Hernando
Jan 7, 2011·Extremophiles : Life Under Extreme Conditions·I V Ambily Nath, P A Loka Bharathi
Jun 2, 2011·Extremophiles : Life Under Extreme Conditions·Xi-Ming ChenLi-Zhe An
Mar 22, 2007·Antonie van Leeuwenhoek·Bernice M TanGeorge L Mendz
Dec 28, 2012·Applied Biochemistry and Biotechnology·Karthikeyan GunasekaranAnwar Sunna
Jan 17, 2012·Protein & Cell·Xiang WangWeimin Gong
Apr 28, 2004·The International Journal of Biochemistry & Cell Biology·T O LukashA V El'skaya
Jun 14, 2002·Archives of Biochemistry and Biophysics·Gisela KramerB Hardesty
Feb 1, 2003·Journal of Molecular Biology·Brian Søgaard LaursenHans Uffe Sperling-Petersen
Dec 5, 1998·Biochimica Et Biophysica Acta·I M Krab, A Parmeggiani
Nov 19, 2003·International Journal of Food Microbiology·Fatma MihoubFrançois Billaux
Feb 19, 2000·Current Opinion in Structural Biology·D E Feldman, J Frydman
Jan 17, 2012·Nature Structural & Molecular Biology·Daijiro Takeshita, Kozo Tomita
Aug 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Daijiro Takeshita, Kozo Tomita
Sep 19, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Jing QiYuqing Liu
Sep 10, 2004·European Journal of Biochemistry·Damodara RaoZ Ristic
Mar 28, 2002·Applied and Environmental Microbiology·Ophélie DuchéJean Labadie
Sep 6, 2003·Applied and Environmental Microbiology·Joanna C WilkinsKaren A Homer
Apr 16, 2008·Infection and Immunity·Sowmya BalasubramanianJoel B Baseman
Sep 16, 2008·Journal of Bacteriology·Sandra IsabelMichel G Bergeron
May 28, 2005·Canadian Journal of Microbiology·M A HeffordM L Kalmokoff
Apr 16, 2011·Canadian Journal of Microbiology·Selim CeylanDilek Kazan

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