Chaperoning RPA during DNA metabolism

Current Genetics
Shuqi LiQing Li

Abstract

Single-stranded DNA (ssDNA) is widely generated during DNA metabolisms including DNA replication, repair and recombination and is susceptible to digestion by nucleases and secondary structure formation. It is vital for DNA metabolism and genome stability that ssDNA is protected and stabilized, which are performed by the major ssDNA-binding protein, and replication protein A (RPA) in these processes. In addition, RPA-coated ssDNA also serves as a protein-protein-binding platform for coordinating multiple events during DNA metabolisms. However, little is known about whether and how the formation of RPA-ssDNA platform is regulated. Here we highlight our recent study of a novel RPA-binding protein, Regulator of Ty1 transposition 105 (Rtt105) in Saccharomyces cerevisiae, which regulates the RPA-ssDNA platform assembly at replication forks. We propose that Rtt105 functions as an "RPA chaperone" during DNA replication, likely also promoting the assembly of RPA-ssDNA platform in other processes in which RPA plays a critical role.

References

Jul 1, 1992·Molecular and Cellular Biology·C KimM S Wold
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M S Wold, T Kelly
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C R WobbeJ Hurwitz
Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·P A Bullock
Aug 12, 1998·Nature Structural Biology·T IkegamiM Shirakawa
Apr 10, 1999·Genes & Development·W L de LaatJ H Hoeijmakers
Sep 3, 1999·Critical Reviews in Biochemistry and Molecular Biology·C IftodeJ A Borowiec
Nov 29, 2002·The EMBO Journal·Nima MosammaparastLucy F Pemberton
Feb 13, 2003·Current Opinion in Structural Biology·Christopher W Akey, Karolin Luger
Jul 26, 2003·The Journal of Biological Chemistry·Alphonse I ArunkumarWalter J Chazin
Sep 16, 2004·Molecular and Cellular Biology·Deena M LeslieJohn D Aitchison
Aug 29, 2006·Nucleic Acids Research·Ellen FanningAndrew R Nager
Feb 17, 2007·Experimental and Molecular Pathology·Christophe BraetHeinz Peter Nasheuer
Dec 25, 2007·Cell Research·Guo-Min Li
Feb 16, 2008·Annual Review of Biochemistry·Joseph San FilippoHannah Klein
May 13, 2008·The Journal of Biological Chemistry·Stuart J HaringMarc S Wold
Apr 22, 2009·Journal of the American Chemical Society·Chris A BroseyWalter J Chazin
Jun 3, 2010·Critical Reviews in Biochemistry and Molecular Biology·Rachel Litman Flynn, Lee Zou
Oct 30, 2010·Biochimica Et Biophysica Acta·Yuh Min Chook, Katherine E Süel
Sep 14, 2011·Annual Review of Genetics·Lorraine S Symington, Jean Gautier
Oct 22, 2011·Biochimica Et Biophysica Acta·Kristin M Keck, Lucy F Pemberton
Mar 16, 2012·Genetics·John D Aitchison, Michael P Rout
Apr 3, 2012·Nucleic Acids Research·Lumir KrejciXiaolan Zhao
Oct 17, 2012·Genes & Development·Jie Fan, Nikola P Pavletich
Jan 5, 2013·Nature Structural & Molecular Biology·Rebecca J Burgess, Zhiguo Zhang
Jan 11, 2013·Nucleic Acids Research·Chris A BroseyWalter J Chazin
Jun 7, 2014·Annual Review of Biochemistry·Zachary A Gurard-LevinGeneviève Almouzni

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Citations

Aug 26, 2020·FEMS Microbiology Reviews·Matan ArbelMartin Kupiec
Jul 25, 2019·Current Genetics·Soumitra Sau, Martin Kupiec
May 13, 2020·Current Genetics·Matan ArbelMartin Kupiec
Mar 20, 2020·Cell & Bioscience·Wenshuo ZhangQing Li

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Methods Mentioned

BETA
immunoprecipitation
pull-down
electrophoretic mobility shift assay
X-ray
electron microscopy

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