Chapter 12: Nanoscale biological fluorescence imaging: breaking the diffraction barrier

Methods in Cell Biology
Travis J Gould, Samuel T Hess

Abstract

Biological imaging has been limited by the finite resolution of light microscopy. Recent developments in ultra-high-resolution microscopy methods, many of which are based on fluorescence, are breaking the diffraction barrier; it is becoming possible to image intracellular protein distributions with resolution of tens of nanometers or better. Fluorescence photoactivation localization microscopy (FPALM) is an example of such an ultra-high-resolution method which can image living or fixed cells with demonstrated lateral resolution of better than 20 nm. A detailed description of the methods involved in FPALM imaging of biological samples is presented here, accompanied by comparison with existing methods from the literature.

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Citations

May 10, 2017·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Erdinc Sezgin
Jan 21, 2014·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Brian R LongHaining Zhong
Nov 9, 2017·Biophysical Journal·Francesca PennacchiettiSamuel T Hess

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