Chapter 13: Imaging of cells and tissues with mass spectrometry: adding chemical information to imaging.

Methods in Cell Biology
Tyler A ZimmermanJonathan V Sweedler

Abstract

Techniques that map the distribution of compounds in biological tissues can be invaluable in addressing a number of critical questions in biology and medicine. One of the newer methods, mass spectrometric imaging, has enabled investigation of spatial localization for a variety of compounds ranging from atomics to proteins. The ability of mass spectrometry to detect and differentiate a large number of unlabeled compounds makes the approach amenable to the study of complex biological tissues. This chapter focuses on recent advances in the instrumentation and sample preparation protocols that make mass spectrometric imaging of biological samples possible, including strategies for both tissue and single-cell imaging using the following mass spectrometric ionization methods: matrix-assisted laser desorption/ionization, secondary ion, electrospray, and desorption electrospray.

Citations

Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Tyler A ZimmermanJonathan V Sweedler
Oct 5, 2011·Analytical Chemistry·Francisco A Fernandez-LimaEmile A Schweikert
Apr 5, 2013·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Maciej LalowskiMarc Baumann
Dec 27, 2011·The American Journal of Dermatopathology·Rossitza LazovaRichard M Caprioli
Nov 19, 2010·Bioanalysis·Richard J A Goodwin, Andrew R Pitt
Nov 26, 2009·Natural Product Reports·Eduardo EsquenaziPieter C Dorrestein
Aug 20, 2010·Nature Reviews. Cancer·Kristina Schwamborn, Richard M Caprioli
Jan 25, 2011·Rapid Communications in Mass Spectrometry : RCM·Stormy L KoenigerStephen Spanton
Nov 1, 2015·Journal of Nanobiotechnology·Monique Culturato Padilha MendonçaMaria Alice da Cruz-Höfling
Dec 29, 2015·Neuromolecular Medicine·Saara TikkaMaciej Lalowski
Jan 29, 2021·Expert Review of Proteomics·Al MamunMitsutoshi Setou
Apr 2, 2021·Expert Review of Proteomics·Md Mahamodun NabiMitsutoshi Setou
Jun 23, 2011·Analytical Chemistry·Tyler A ZimmermanEdwin De Pauw

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