Character, distribution and biological implications of ice crystallization in cryopreserved rabbit ovarian tissue revealed by cryo-scanning electron microscopy

Human Reproduction
R G GosdenG John Morris

Abstract

Ovarian tissue banking is an emerging strategy for fertility preservation which has led to several viable pregnancies after transplantation. However, the standard method of slow cooling was never rigorously optimized for human tissue nor has the extent and location of ice crystals in tissue been investigated. To address this, we used cryo-scanning electron microscopy (cryo-SEM) to study ice formation in cryopreserved ovarian tissue. Rabbit ovarian tissue slices were equilibrated in 1,2-propanediol-sucrose solution and cooled at either 0.3 degrees C/min or 3.0 degrees C/min after nucleating ice at -7 degrees C, or snap-frozen by plunging in liquid nitrogen. Frozen tissues were fractured, etched and coated with gold or prepared by freeze substitution and sectioning for cryo-SEM. The size, location and orientation of extracellular ice crystals were revealed as pits and channels that had grown radially between freeze-concentrated cellular materials. They represented 60% of the total volume in slowly cooled samples that were nucleated at -7 degrees C and the crystals, often >30 microm in length, displaced cells without piercing them. Samples cooled more rapidly were much less dehydrated, accounting for the presence of small ice crys...Continue Reading

References

Sep 1, 1990·Journal of Reproduction and Fertility·J CarrollR G Gosden
Apr 30, 1999·Human Reproduction·G J MorrisS Avery
Jan 27, 2005·Reproductive Biomedicine Online·Barry Fuller, Sharon Paynter
Mar 24, 2005·Journal of the National Cancer Institute. Monographs·Roger G Gosden
Jul 20, 2006·Human Reproduction Update·Jacques DonnezMarie-Madeleine Dolmans
Sep 8, 2006·Cell and Tissue Banking·David E Pegg
Sep 1, 2007·Fertility and Sterility·Stefania A NottolaJacques Donnez
Oct 13, 2007·Cryobiology·Vladimir IsachenkoHans van der Ven
Jul 8, 2008·Human Reproduction·Claus Yding AndersenErik Ernst
Feb 28, 2009·The New England Journal of Medicine·Jacqueline S Jeruss, Teresa K Woodruff
Apr 11, 2009·Human Reproduction·Victoria KerosOuti Hovatta
May 15, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·V IsachenkoJ M Weiss

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Citations

Aug 27, 2013·PloS One·Pierre BruyèreSamuel Buff
Jan 15, 2014·Tissue Engineering. Part C, Methods·Isobel MassieG John Morris
Feb 5, 2013·Fertility and Sterility·Julie VanackerAlessandra Camboni
Dec 19, 2012·Cryobiology·G John Morris, Elizabeth Acton
Jul 6, 2010·Cryobiology·Jonghoon KangNathan R Fisher
Jun 17, 2011·Reproductive Biomedicine Online·Christiani A AmorimJacques Donnez
Jan 29, 2011·Biology Letters·Tracy A BedrosianRandy J Nelson
Dec 29, 2019·Scientific Reports·Ellen Cristina Rivas LeonelChristiani A Amorim

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