Apr 1, 2020

Contemporary Enterovirus D68 strains show enhanced replication and translation at 37°C

BioRxiv : the Preprint Server for Biology
B. Smith, Andrew Pekosz


Enterovirus D68 (EV-D68) emerged in 2014 as an important pathogen linked to severe lower respiratory disease and acute flaccid myelitis outbreaks. Historically associated with mild common-cold-like symptoms, clusters of severe disease attributed to EV-D68 appeared during a series of outbreaks in 2014, 2016, and 2018. Previous studies of historic EV-D68 strains demonstrated attenuated replication at temperatures of the lower respiratory tract (37{degrees}C), when compared to the upper respiratory tract (32{degrees}C). By testing a panel of historic and contemporary EV-D68 strains at 32{degrees}C and 37{degrees}C, we demonstrate that contemporary strains of EV-D68 undergo little to no attenuation at increased temperatures. Contemporary strains produced higher levels of viral proteins at 32{degrees}C and 37{degrees}C than historic strains, although both strains infected similar numbers of cells and had comparable amounts of replication complexes. IRES activity assays with dual-luciferase reporter plasmids demonstrated enhanced translation in recent EV-D68 strains mapped to regions of variability in the 5 UTR found only in contemporary strains. Using an infectious clone system, we demonstrate that the translation advantage dictated...Continue Reading

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Mentioned in this Paper

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