Characterisation and comparison of temporal release profiles of nitric oxide generating donors

Journal of Neuroscience Methods
Sophie A Bradley, Joern R Steinert

Abstract

Nitric oxide (NO) is a vital signalling molecule in a variety of tissues including the neuronal, vascular and reproductive system. However, its high diffusibility and inactivation make characterisation of nitrergic signalling difficult. The use of NO donors is essential to characterise downstream signalling pathways but knowledge of donor release capacities is lacking, thus making comparisons of donor responses difficult. This study characterises NO profiles of commonly used NO donors. Donors were stored under defined conditions and temporal release profiles detected to allow determination of released NO concentrations. Using NO-sensitive microsensors we assessed release profiles of NO donors following different storage times and conditions. We found that donors such as NOC-5 and PAPA-NONOate decayed substantially within days, whereas SNP and GSNO showed greater stability releasing consistent levels of NO over days. In all donors tested, the amount of released NO differs between frozen and unfrozen stocks. Fluorescent and amperometric approaches to measure NO concentrations yield a wide range of levels. However, due to a lack of characterisation of the release profiles, inconsistent effects on NO signalling have been widely doc...Continue Reading

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Citations

Mar 30, 2019·The Journal of Physiology·Nicolas R ChevalierOrestis Faklaris
Dec 5, 2015·Oxidative Medicine and Cellular Longevity·Sophie A Bradley, Joern R Steinert
Nov 11, 2017·Polymers·George FlemingRaechelle A D'Sa
Sep 20, 2019·Antioxidants·Emily Hays, Benjamin Bonavida
Nov 18, 2020·International Journal of Molecular Sciences·Marlena ZyśkTadeusz Pawełczyk
Nov 23, 2021·Channels·Jereme G Spiers, Joern R Steinert

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