Characterisation and identification of proteinaceous binding media (animal glues) from their amino acid profile by capillary zone electrophoresis

Journal of Separation Science
Isabella KamlErnst Kenndler

Abstract

A capillary electrophoretic method for identifying different species of proteinaceous binders--collagen, egg white, and milk casein--is described. It allows characterisation of the proteins on the basis of the amino acid profiles obtained after their acidic hydrolysis. The profiles of the underivatised amino acids are recorded directly by capillary zone electrophoresis at pH 2.26 with the aid of a conductivity detector, thus eliminating the need for a derivatisation step. Identification is carried out by means of different relative peak areas of the amino acids. A scheme is given for identification, which is based on the main markers, hydroxyproline, proline, glycine, glutamic acid, and serine and valine.

References

Dec 5, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·S Oguri
May 24, 2001·Fresenius' Journal of Analytical Chemistry·R Mateo-CastroM T Doménech-Carbó
Feb 5, 2002·Electrophoresis·C PrataF Couderc
May 16, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jetse C ReijengMarcella Chiari
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gordana ZunićSlavica Spasić

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Citations

Dec 29, 2015·Chemical Reviews·Sophie DallongevilleCaroline Tokarski
Sep 15, 2006·Journal of Separation Science·Veronika Solínová, Václav Kasicka
Feb 23, 2007·Journal of Separation Science·Petr PrikrylPetr Hofta
Mar 21, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ivan MiksíkAdam Eckhardt
Jun 25, 2008·Analytica Chimica Acta·María Teresa Doménech-Carbó

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