Characterisation and immunosuppressive activity of human cartilage-derived mesenchymal stem cells

Cytotechnology
Pratheep SandrasaigaranRajesh Ramasamy

Abstract

Mesenchymal stem cells (MSCs) exert potent immuno-regulatory activities on various immune cells and also differentiate into various mesodermal lineages besides retaining a distinct self-renewal ability. Such exclusive characteristics had enabled MSCs to be recognised as an ideal source for cell-based treatment in regenerative medicine and immunotherapy. Thus, considering MSCs for treating degenerative disease of organs with limited regenerative potential such as cartilage would serve as an ideal therapy. This study explored the feasibility of generating human cartilage-derived MSCs (hC-MSCs) from sports injured patients and characterised based on multipotent differentiation and immunosuppressive activities. Cartilage tissues harvested from a non-weight bearing region during an arthroscopy procedure were used to generate MSCs. Despite the classic morphology of fibroblast-like cells and a defined immunophenotyping, MSCs expressed early embryonic transcriptional markers (SOX2, REX1, OCT4 and NANOG) and differentiated into chondrocytes, adipocytes and osteocytes when induced accordingly. Upon co-culture with PHA-L activated T-cells, hC-MSCs suppressed the proliferation of the T-cells in a dose-dependent manner. Although, hC-MSCs di...Continue Reading

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Citations

Feb 25, 2020·Journal of Biomedical Materials Research. Part a·Hongyi LiuYilin Zhao
Aug 9, 2018·International Journal of Molecular Sciences·Timo GaberPaula Hoff
Oct 14, 2020·Stem Cells and Development·Zhihua RenRiyi Shi

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