Characterisation and partial purification of a novel prohormone processing enzyme from ovine adrenal medulla

FEBS Letters
N Tezapsidis, D C Parish

Abstract

An enzymatic activity has been identified which is capable of generating a product chromatographically identical with adrenorphin from the model substrate BAM12P. This enzyme was purified by gel filtration and ion-exchange chromatography and characterised as having a molecular mass between 30 and 45 kDa and an acidic pI. The enzyme is active at the acid pH expected in the secretory vesicle interior and is inhibited by EDTA, suggesting that it is a metalloprotease. This activity could not be mimicked by incubation with lysosomal fractions and it meets the criteria to be considered as a possible prohormone processing enzyme.

References

Jul 1, 1988·Journal of Biochemistry·A MoriyamaM Sasaki
Oct 15, 1986·Biochemical and Biophysical Research Communications·J SakataH Matsuo
Feb 29, 1984·Biochemical and Biophysical Research Communications·E F WallaceC J Evans
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E WeberC J Evans
Jan 1, 1984·Annual Review of Neuroscience·Y P LohH Gainer
Aug 16, 1984·Biochemical and Biophysical Research Communications·M SondersE Weber
Aug 12, 1982·Nature·A F BradburyD G Smyth

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Citations

Jul 22, 1991·Biochimica Et Biophysica Acta·W J Lennarz, W J Strittmatter
Feb 1, 1990·Bioscience Reports·N J Darby, D G Smyth
Oct 6, 1995·The Journal of Biological Chemistry·Y L BermanL A Devi

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