PMID: 9428673Jan 15, 1998Paper

Characterisation and properties of an intracellular lipid-binding protein from the tapeworm Moniezia expansa

European Journal of Biochemistry
John BarrettPeter M Brophy

Abstract

The tapeworm Moniezia expansa contains an extremely abundant cytoplasmic lipid-binding protein (LBP). It is a small protein consisting of 66 amino acids with a molecular mass of 7943 +/- 1.5 Da. The amino acid sequence has been established by Edman degradation and confirmed by PCR analysis. The Moniezia LBP shows no sequence similarity with any previously described binding protein, but does show similarity with antigen B from Echinococcus glanulosus and Echinococcus multilocularis and with Taenia crassiceps antigen. The predicted structure for Moniezia LBP shows four helices and a putative tyrosine kinase site on the loop between helix 1 and 2. Each of the four helices has a well defined hydrophobic face. Studies with fluorescent probes suggest a single hydrophobic binding site. Results indicate that the single tryptophan residue in the molecule (Trp41) is involved in ligand binding, and calculation of the Stern-Volmer quenching constant shows that Trp41 is in a relatively hydrophobic environment.

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Citations

Aug 28, 2001·Molecular and Biochemical Parasitology·G ChemaleA Zaha
Aug 22, 2000·Parasitology Today·M W Kennedy
Mar 7, 2009·Parasitology·J Barrett
Jul 11, 2012·Parasitology·Peter M BrophyRussell M Morphew
Apr 24, 1999·European Journal of Biochemistry·A TimanovaR D Walter
May 12, 2001·Infection and Immunity·A LoukasD P McManus
Jan 1, 1999·Bioscience, Biotechnology, and Biochemistry·F EilbertH Anke
May 23, 2012·PLoS Neglected Tropical Diseases·Gonzalo ObalAna María Ferreira
Feb 9, 2005·Biochimica Et Biophysica Acta·Gustavo ChemaleArnaldo Zaha
Dec 3, 2014·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Valeria Silva-ÁlvarezAna María Ferreira
Nov 11, 2009·International Journal for Parasitology·Lisa PiersonAaron G Maule
Jun 18, 2005·BMC Bioinformatics·Liisa B KoskiGertraud Burger
Sep 15, 2006·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·S L HotiP K Das
Dec 15, 2005·Parasitology International·Yasuhito SakoAkira Ito
Apr 5, 2017·Annual Review of Biochemistry·Lucy MalininaRhoderick E Brown
Apr 19, 2008·Amino Acids·Zhi-Ping LiuLuonan Chen
Sep 21, 2005·Prostate Cancer and Prostatic Diseases·Y KinoshitaG P Haas
Mar 24, 2006·World Journal of Surgery·Yoshihisa KinoshitaGabriel P Haas
Aug 27, 2015·AMB Express·John Selesteen Charles Adlin JeniferThavasimuthu Citarasu
May 5, 2018·PLoS Neglected Tropical Diseases·Edileuza Danieli da SilvaArnaldo Zaha
Feb 12, 2000·Biochimica Et Biophysica Acta·M W Kennedy

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