Characterisation of a cellular model of cardiomyopathy, in the rabbit, produced by chronic administration of the anthracycline, epirubicin

Journal of Molecular and Cellular Cardiology
E J KelsoB Silke

Abstract

The objective of this study was to assess the structural, mechanical and electrophysiological changes associated with chronic administration of epirubicin (Pharmorubicin), which is a cardiotoxic anthracycline antibiotic used in conventional cancer chemotherapy. New Zealand white rabbits (8 weeks) were treated with twice-weekly infusions of epirubicin (2 mg/kg) or saline for a period of 6 weeks, followed by a wash-out period of 2 weeks. Myocardial damage consistent with cardiomyopathy was observed in the epirubicin-treated animals; electron micrographs indicated myofibril loss together with separation of the intercalated disc and dilation of the sarcotubular system. Contractile function, as measured by mechanical shortening, action potentials and L-type Ca2+ currents were examined in ventricular cardiomyocytes, which were isolated by means of enzymatic dissociation using collagenase. There was an attenuation in the contractile response to isoprenaline in cardiomyocytes isolated from the hearts of epirubicin-treated rabbits compared to control rabbits. Cardiomyocytes isolated from epirubicin-treated rabbits had greater basal contractile amplitude (11.0+/-0.3 %dL, n=8) than control myocytes (8.2+/-0.3 %dL, n=9), but had similar ma...Continue Reading

Citations

Oct 15, 2008·Cardiovascular Research·Naranjan S DhallaParamjit S Tappia
Nov 19, 2009·International Journal of Cardiology·Arie O VerkerkRuben Coronel
Nov 21, 2015·Journal of cardiology·Tomasz A BondaKarol A Kamiński
Jul 23, 2005·Free Radical Research·Mireille MousseauAlain Favier
May 19, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·F Muders, D Elsner
Feb 4, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Luca SantucciStefano Fiorucci
Nov 16, 2019·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Juthipong BenjanuwattraNipon Chattipakorn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Electrophysiology

Cardiac electrophysiology is the study of electrical activities of the heart and includes the assessment, diagnosis, and treatment of cardiac events. Find the latest research on cardiac electrophysiology here.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.