PMID: 8950188Nov 11, 1996Paper

Characterisation of a DNA pairing activity copurifying with DNA ligase in a partially purified extract from rat testis

Biochimica Et Biophysica Acta
S AcharyaM R Rao

Abstract

Rat testicular nuclear extracts were fractionated sequentially on phosphocellulose, heparin-agarose and ssDNA-cellulose columns, in order to isolate and characterise a strand-transfer activity from a mammalian meiotic tissue. A partially purified fraction, eluting at 0.6 M KCl from ssDNA-cellulose column, catalyzed the formation of two classes of products migrating slowly on an agarose gel. The formation of one of these classes of products-the aggregates-was dependent on the presence of both the substrates (M13mp19 RF III and M13mp19 ssDNA) and on homology. The presence of ATP was essential for the formation of aggregates, though its hydrolysis was not required. EM analysis of the products indicated the presence of structures which resembled paired DNA molecules: duplex-duplex paired (Y-shaped and ds-ds paired structures) and ss-ds paired (duplex DNA paired with the single-stranded DNA) structures, indicating the presence of a pairing protein in the fraction. However, alpha- and sigma-structures were not observed. The other class of products, seen as discrete bands, were identified biochemically and by electron microscopy as ligated products. A DNA ligase-adenylate adduct of molecular weight 100 kDa was formed by the fraction. ...Continue Reading

References

Sep 25, 1977·Journal of Molecular Biology·C Christiansen, R L Baldwin
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J H Wilson
Jan 1, 1976·Annual Review of Genetics·B S BakerL Sandler
Jan 1, 1977·Methods in Cell Biology·R D PlatzS R Grimes
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M S Meselson, C M Radding
Jan 1, 1992·Annual Review of Biochemistry·S C West
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S P MooreR Fishel
Mar 1, 1991·Molecular and Cellular Biology·B A Ozenberger, G S Roeder
Jan 23, 1989·Journal of Theoretical Biology·S McGavin
Jan 1, 1988·Annual Review of Genetics·D S Thaler, F W Stahl
Mar 1, 1985·Microbiological Reviews·T L Orr-Weaver, J W Szostak
Sep 20, 1985·Science·H BernsteinR E Michod
Mar 1, 1985·Scientific American·C M Croce, G Klein
Jan 1, 1982·Annual Review of Genetics·C M Radding
Apr 14, 1983·Nature·S Tonegawa
Mar 1, 1984·Cell·E B Kmiec, W K Holloman
May 1, 1983·Cell·J W SzostakF W Stahl
Jan 1, 1984·Annual Review of Biochemistry·G R Stark, G M Wahl
Jan 24, 1981·Nucleic Acids Research·M M Cox, I R Lehman
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M M Cox, I R Lehman
Jan 1, 1981·Annual Review of Genetics·S A Latt
May 1, 1994·Trends in Biochemical Sciences·M M Cox
Jan 1, 1993·Progress in Nucleic Acid Research and Molecular Biology·W D Heyer, R D Kolodner
Jan 1, 1994·Molecular and Cellular Biology·M K DerbyshireR Fishel

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.