PMID: 7538249Feb 1, 1995Paper

Characterisation of a membrane receptor on ruminants and equine platelets and peripheral blood leukocytes similar to the human integrin receptor glycoprotein IIb/IIIa (CD41/61)

Veterinary Immunology and Immunopathology
C O PintadoD Llanes

Abstract

This paper describes two anti-glycoprotein IIb/IIIa or CD41/61 murine monoclonal antibodies (Co.35E4 and Co.2oA1). The cellular distribution and apparent molecular weight of the antigen detected by these antibodies is consistent with their reaction with ruminant and equine glycoprotein IIb/IIIa. Biochemical analysis of the equine molecule using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) revealed bands of 24, 100 and 110 kDa under reducing conditions and 115 and 80 kDa under nonreducing conditions. Biochemical analysis of ruminant antigen revealed that the 24 kDa band did not appear owing to the absence of labelling with sulpho-NHS-biotin. Co.35E4 and Co.20A1 recognised two different Ca2+ complex independent epitopes. The glycoprotein IIb/IIIa was present on ruminant and equine granulocytes, monocytes and platelets. However, binding on granulocytes and monocytes was due to the adsorption of membrane platelet fragments.

Citations

Aug 1, 1995·Veterinary Immunology and Immunopathology·C O Pintado, D Llanes
Aug 1, 1996·Veterinary Immunology and Immunopathology·A MateoD Llanes
Aug 1, 1996·Veterinary Immunology and Immunopathology·A MateoD Llanes
Aug 1, 1996·Veterinary Immunology and Immunopathology·J M Pérez de la LastraD Llanes
Aug 1, 1996·Veterinary Immunology and Immunopathology·M SimonS Hluchy
Mar 2, 1999·Comparative Immunology, Microbiology and Infectious Diseases·A MateoD Llanes
Jan 25, 2003·Veterinary Immunology and Immunopathology·Cory C LalkoBenjamin J Darien
Jun 25, 1998·Veterinary Immunology and Immunopathology·D P LunnK E Vagnoni
Apr 5, 2013·PloS One·Po-Kong ChenDer-Shan Sun
Oct 14, 2014·Veterinary Surgery : VS·Caitlin M O'SheaLinda A Dahlgren
Mar 14, 2003·Journal of Comparative Pathology·A PelagalliN Staiano

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