PMID: 2105219Jan 26, 1990Paper

Characterisation of a monoclonal antibody against native human type I collagen

European Journal of Biochemistry
J A WerkmeisterG Ellender

Abstract

A monoclonal antibody against a pepsin-soluble mammalian type I collagen has been produced. This antibody, subclass IgG1, kappa, was specific for type I collagen and did not cross-react with a range of other collagen types or connective tissue proteins. The epitope recognized by the antibody was dependent upon an intact triple-helical structure for the collagen, and was shown by rotary shadowing and by immunoblotting of collagenase-derived fragments to be near the C-terminal of the pepsin-soluble collagen. Although the antibody had a low affinity, with Kd = 4 x 10(-7) M, it could be used for immunohistology of tissue sections and for studies of collagen produced by cells in culture. The antibody, which was raised against human collagen, also recognized type I collagens from certain other species, including calf, pig, sheep, goat and dog.

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Citations

Jun 1, 1991·International Journal of Biological Macromolecules·V GlattauerJ A Werkmeister
Mar 31, 2007·Reproduction, Fertility, and Development·Veronica GlattauerJohn A M Ramshaw
May 1, 1996·Australian and New Zealand Journal of Ophthalmology·B E OrsidaR H West
Jan 1, 1997·Journal of Biomaterials Science. Polymer Edition·C Shanthi, K P Rao
Oct 6, 2012·Biomaterials·Yen B TruongJohn A M Ramshaw
Jan 8, 2010·Biomaterials·Anna V TaubenbergerDietmar W Hutmacher
Nov 19, 2013·Journal of Biomedical Materials Research. Part a·Jacinta F WhiteJohn A M Ramshaw
Sep 25, 2009·Journal of Biomedical Materials Research. Part a·Yong Y PengJerome A Werkmeister
Dec 9, 1992·Clinical Materials·J F WhiteJ A Ramshaw
Oct 1, 2014·Journal of Biomedical Materials Research. Part a·Aditya V VashiJerome A Werkmeister
Jun 18, 2002·Journal of Structural Biology·Jacinta F WhiteJohn A M Ramshaw
Jul 30, 2014·Frontiers in Psychology·Liliana Albertazzi, Roberto Poli
Sep 1, 1997·The Journal of Urology·H P KooE J Macarak
Sep 29, 1998·The Journal of Urology·C M DeveaudP S Howard
May 15, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sok-Siam GoukWendell Q Sun
Aug 1, 1992·The Journal of Urology·D H EwaltE J Macarak
Mar 5, 1997·Journal of Biomedical Materials Research·G A EdwardsJ A Ramshaw
Jan 1, 1993·The Journal of Urology·L S BaskinE J Macarak
Jan 2, 2021·Bioengineering·James D San AntonioJoseph P R O Orgel
Jun 23, 2012·Journal of the American Chemical Society·Arjun S AdhikariAlexander R Dunn

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