Characterisation of a new rubredoxin isolated from Desulfovibrio desulfuricans 27774: definition of a new family of rubredoxins

FEBS Letters
J LeGallMiguel Teixeira

Abstract

A new rubredoxin from the sulphate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774, grown with nitrate as terminal electron acceptor, was isolated and characterised. The protein is an 8.5 kDa monomer containing one iron atom per molecule, with a reduction potential of 25 +/- 5 mV at pH 7.6. Like the recombinant Rdl protein from D. vulgaris, expressed in Escherichia coli [Lumpio, H.L., Shenvi, N.V., Garg, R.P., Summers, A.O. and Kurtz, D.M., J. Bacteriol. 179 (1997) 4607-4615], it contains an unusual spacing of four amino acids between the first two of the iron coordinating cysteinyl residues. This difference is reflected in the structure of the iron centre, as observed by visible and EPR spectroscopies. All together, these features make these proteins the first members of a new family of rubredoxins.

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Citations

Aug 26, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Françoise AuchèreJosé J G Moura
Mar 16, 2005·Biochemical and Biophysical Research Communications·João V RodriguesMiguel Teixeira
Apr 24, 1999·European Journal of Biochemistry·C V RomãoM Teixeira
Jun 25, 2005·Biochemical and Biophysical Research Communications·Bárbara J HenriquesCláudio M Gomes
Oct 27, 2016·Journal of Molecular Biology·Célia V RomãoCarlos Frazão
Feb 18, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Isabel A AbreuLígia M Saraiva
Sep 29, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Jamie L MichalekSarah L J Michel
Jul 11, 2021·Environmental Microbiology·Claire MorvanIsabelle Martin-Verstraete

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