Characterisation of a newly detected bacteriophage infecting Bordetella bronchiseptica in swine

Archives of Virology
Yibao ChenBin Wu

Abstract

A novel virulent bacteriophage, vB_BbrM_PHB04, infecting Bordetella bronchiseptica was isolated from wastewater collected at a swine farm in China. Phage vB_BbrM_PHB04 exhibited growth over a wide range of temperature and pH conditions and showed different efficiency of plating values and lytic spectra within the same strains at 25 °C and 37 °C. High-throughput sequencing revealed that vB_BbrM_PHB04 has a linear double-stranded DNA genome with 124 putative open reading frames. Overall, the genome of vB_BbrM_PHB04 showed very low similarity (the highest nucleotide identity 82%, 1% coverage) to other phage sequences in the GenBank database. Phylogenetic analysis indicated that vB_BbrM_PHB04 is a new member of the family Myoviridae. In addition, polymerase chain reaction-based detection of phage genes in phage-resistant B. bronchiseptica variants revealed no evidence of lysogenic activity of phage vB_BbrM_PHB04.

References

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Jan 25, 2005·Infection and Immunity·Nuria Vergara-IrigarayGuillermo Martínez de Tejada
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Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
May 8, 2014·Mediterranean Journal of Hematology and Infectious Diseases·Abraham T YacoubJohn Greene
Nov 25, 2014·Applied and Environmental Microbiology·Yasuhiko KawatoToshihiro Nakai
May 6, 2016·Frontiers in Microbiology·Carlota BardinaMontserrat Llagostera

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Methods Mentioned

BETA
PCR

Software Mentioned

SOAPdenovo
BLAST
tRNAscan
ClustalW
Glimmer
MEGA
MEGA6
SE

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