Characterisation of an Isogenic Model of Cisplatin Resistance in Oesophageal Adenocarcinoma Cells

Pharmaceuticals
Amy M BuckleyJacintha O'Sullivan

Abstract

Cisplatin (cis-diamminedichloroplatinum) is widely used for the treatment of solid malignancies; however, the development of chemoresistance hinders the success of this chemotherapeutic in the clinic. This study provides novel insights into the molecular and phenotypic changes in an isogenic oesophageal adenocarcinoma (OAC) model of acquired cisplatin resistance. Key differences that could be targeted to overcome cisplatin resistance are highlighted. We characterise the differences in treatment sensitivity, gene expression, inflammatory protein secretions, and metabolic rate in an isogenic cell culture model of acquired cisplatin resistance in OAC. Cisplatin-resistant cells (OE33 Cis R) were significantly more sensitive to other cytotoxic modalities, such as 2 Gy radiation (p = 0.0055) and 5-fluorouracil (5-FU) (p = 0.0032) treatment than parental cisplatin-sensitive cells (OE33 Cis P). Gene expression profiling identified differences at the gene level between cisplatin-sensitive and cisplatin-resistant cells, uncovering 692 genes that were significantly altered between OE33 Cis R cells and OE33 Cis P cells. OAC is an inflammatory-driven cancer, and inflammatory secretome profiling identified 18 proteins secreted at significant...Continue Reading

References

Aug 15, 1996·The New England Journal of Medicine·T N WalshT P Hennessy
May 22, 2002·International Journal of Radiation Biology·W K MyintG P Raaphorst
Dec 31, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·David J AdelsteinArlene A Forastiere
May 12, 2005·Molecular Cancer Research : MCR·Heather J BoeckmanJohn J Turchi
Jul 11, 2006·The New England Journal of Medicine·David Cunningham MAGIC Trial Participants
Apr 28, 2007·Cell Death and Differentiation·P VlachosB Joseph
Sep 22, 2007·IUBMB Life·Britta Stordal, Mary Davey
Jul 29, 2008·Current Genomics·Feng-Qi Zhao, Aileen F Keating
Dec 17, 2009·World Journal of Gastroenterology : WJG·Hai-Lin JinRui-Hua Shi
Jan 20, 2011·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Richard HummelDamian J Hussey
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Sep 6, 2011·Oncogene·L GalluzziG Kroemer
Mar 15, 2012·Cancer Biology & Therapy·Lei CuiZhong-Ping Chen
Jun 1, 2012·The New England Journal of Medicine·P van HagenUNKNOWN CROSS Group
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Oct 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Peng GuoDebra T Auguste
Oct 14, 2014·Metallomics : Integrated Biometal Science·Nasma D EljackRonald J Clarke
Oct 31, 2014·World Journal of Gastroenterology : WJG·Richard HummelDamian J Hussey
Feb 5, 2015·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Jonathan Cools-LartigueLorenzo E Ferri
Jan 1, 2014·Frontiers in Molecular Biosciences·Rajamanickam BaskarKheng-Wei Yeoh
Jul 24, 2015·Scientific Reports·Hui-Mei WuRong-Yu Liu
Feb 11, 2016·World Journal of Clinical Oncology·Deepak KilariEric S Kim
Jul 21, 2016·Nature Immunology·Nelson C Di Paolo, Dmitry M Shayakhmetov
Mar 10, 2017·World Journal of Gastroenterology : WJG·Xiao-Que XieKang-Sheng Gu
Apr 28, 2017·Annals of Cardiothoracic Surgery·Ghulam Abbas, Mark Krasna
Jul 7, 2017·Cell Metabolism·Gabriela Andrejeva, Jeffrey C Rathmell
Jul 18, 2018·International Journal of Oncology·Yunjie XuLiankun Sun

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Citations

Aug 19, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Philippe MarchettiJérôme Kluza
Sep 19, 2019·Pharmaceuticals·Mary J Meegan, Niamh M O'Boyle
Mar 5, 2021·Biological Chemistry·Nina BaumgartenMarcel H Schulz

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Methods Mentioned

BETA
X-ray
enzyme linked immunosorbent assay
Assay
Illumina sequencing
ELISA

Software Mentioned

pheatmap
R
Cufflinks
EASE ( Expression Analysis Systematic Explorer )
GraphPad
GraphPad Prism

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