PMID: 7545810Jun 1, 1995Paper

Characterisation of Ca(2+)-dependent inwardly rectifying K+ currents in HeLa cells

Pflügers Archiv : European journal of physiology
M Díaz, F V Sepúlveda

Abstract

The whole-cell configuration of the patch-clamp technique was used to examine K+ currents in HeLa cells. Under quasi-physiological ionic gradients, using an intracellular solution containing 10(-7) mol/l free Ca2+, mainly outward currents were observed. Large inwardly rectifying currents were elicited in symmetrical 145 mmol/l KCl. Replacement of all extracellular K+ by isomolar Na+, greatly decreased inward currents and shifted the reversal potential as expected for K+ selectivity. The inwardly rectifying K+ currents exhibited little or no apparent voltage dependence within the range of from -120 mV to 120 mV. A square-root relationship between chord conductance and [K+] at negative potentials could be established. The inwardly rectifying nature of the currents was unaltered after removal of intracellular Mg2+ and chelation with ATP and ethylenediaminetetraacetic acid (EDTA). Permeability ratios for other monovalent cations relative to K+ were: K+ (1.0) > Rb+ (0.86) > Cs+ (0.12) > Li (0.08) > Na+ (0.03). Slope conductance ratios measured at -100 mV were: Rb+ (1.66) > K+ (1.0) > Na+ (0.09) > Li (0.08) > Cs+ (0.06). K+ conductance was highly sensitive to intracellular free Ca2+ concentration. The relationship between conductance...Continue Reading

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Citations

Apr 11, 2006·Pflügers Archiv : European journal of physiology·Patrick Bader, Robert Weingart
Jul 3, 2013·Pflügers Archiv : European journal of physiology·Carolina AñazcoMaría Isabel Niemeyer
Jan 30, 2002·The Journal of General Physiology·Virginijus Valiunas
Jan 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·María Isabel NiemeyerFrancisco V Sepúlveda
Oct 17, 2002·The Journal of Physiology·Diego VarelaFrancisco V Sepúlveda
Jan 16, 2004·The Journal of Physiology·Leandro ZúñigaFrancisco V Sepúlveda
Mar 31, 2017·Scientific Reports·María Isabel NiemeyerFrancisco V Sepúlveda
Oct 1, 2004·The Journal of Membrane Biology·P Bader, R Weingart
Sep 19, 2001·The Journal of Biological Chemistry·M I NiemeyerF V Sepúlveda
Sep 14, 1999·The American Journal of Physiology·A StutzinF V Sepúlveda
Sep 26, 2000·American Journal of Physiology. Cell Physiology·L P CidF V Sepúlveda
Sep 12, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Marcelo CatalánL Pablo Cid
Jul 1, 1997·The American Journal of Physiology·G RiquelmeF V Sepúlveda

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