Characterisation of carbonic anhydrase in Plasmodium falciparum

International Journal for Parasitology
Sudaratana R KrungkraiJerapan Krungkrai

Abstract

Here we report the existence, purification and characterisation of carbonic anhydrase in Plasmodium falciparum. The infected red cells contained carbonic anhydrase approximately 2 times higher than those of normal red cells. The three developmental forms of the asexual stages, ring, trophozoite and schizont were isolated from their host red cells and found to have stage-dependent activity of the carbonic anhydrase. The enzyme was purified to homogeneity from the crude extract of P. falciparum using multiple steps of fast liquid chromatographic techniques. It had a Mr of 32 kDa and was active in a monomeric form. The human red cell enzyme was also purified for comparison with the parasite enzyme. The parasite enzyme activity was sensitive to well-known sulfonamide-based inhibitors of both bacterial and mammalian enzymes, sulfanilamide and acetazolamide. The kinetic properties and the amino terminal sequences of the purified enzymes from the parasite and host red cell were found to be different, indicating that the purified protein most likely exhibited the P. falciparum carbonic anhydrase activity. In addition, the enzyme inhibitors had antimalarial effect against in vitro growth of P. falciparum. Moreover, the vital contributio...Continue Reading

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Citations

Feb 22, 2008·Biological Trace Element Research·Hakan SoyutOlcay Hisar
Jun 1, 2011·Asian Pacific Journal of Tropical Biomedicine·Sudaratana R Krungkrai, Jerapan Krungkrai
Jan 13, 2011·Malaria Journal·Marylin Torrentino-MadametDaniel Parzy
Sep 10, 2014·Journal of Enzyme Inhibition and Medicinal Chemistry·Reza Zolfaghari EmamehSeppo Parkkila
Feb 14, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Sonia Del PreteClemente Capasso
Jun 30, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Ozen Ozensoy GulerClaudiu T Supuran
Jun 26, 2014·Journal of Enzyme Inhibition and Medicinal Chemistry·Hülya GöçerClaudiu T Supuran
Jul 25, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Leyla Polat KoseClaudiu T Supuran
Oct 12, 2011·Journal of Enzyme Inhibition and Medicinal Chemistry·Ramazan DemirdagOmer Irfan Kufrevioglu
Mar 16, 2013·Expert Opinion on Therapeutic Patents·Clemente Capasso, Claudiu T Supuran
Jun 5, 2016·Asian Pacific Journal of Tropical Medicine·Sudaratana R Krungkrai, Jerapan Krungkrai
Sep 13, 2016·Journal of Enzyme Inhibition and Medicinal Chemistry·Sonia Del PreteClemente Capasso
Sep 29, 2011·Toxicology and Industrial Health·Hakan Söyüt, Sükrü Beydemir
Mar 12, 2004·Bioorganic & Medicinal Chemistry Letters·Silvia PastorekovaClaudiu T Supuran
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Oct 27, 2004·Journal of Enzyme Inhibition and Medicinal Chemistry·Sutarnthip ReungprapavutJerapan Krungkrai
Feb 2, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·Deniz EkinciO Irfan Küfrevioğlu
Oct 27, 2004·Journal of Enzyme Inhibition and Medicinal Chemistry·Silvia PastorekovaClaudiu T Supuran
Sep 29, 2004·Journal of Enzyme Inhibition and Medicinal Chemistry·Sükrü Beydemir, Ilhami Gülçin
Mar 27, 2003·Parasitology Research·Miriam KrugliakHagai Ginsburg
Aug 4, 2015·Expert Opinion on Therapeutic Targets·Clemente Capasso, Claudiu T Supuran
Mar 20, 2003·Bioorganic & Medicinal Chemistry Letters·Daniela VulloClaudiu T Supuran
Oct 28, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Siriporn PongsomboonAnchalee Tassanakajon

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