PMID: 8611427Apr 1, 1996Paper

Characterisation of circulating chromogranin A in human cancer patients

British Journal of Cancer
A CortiA G Siccardi

Abstract

The structure of circulating chromogranin A (CgA) of phaeochromocytoma patients was characterised and compared with that of CgA extracted from tumours. Size exclusion chromatography experiments provided evidence that CgA is present in the blood of different patients, as well as in tumour extracts, as multiple forms having different hydrodynamic sizes of 600 kDa (CgA-I), 100 kDa (CgA-II) and 55 kDA (CgA-III). The amount of each CgA form as a proportion of the total antigenic material was different in different patients. Western blot analysis of chromatographic fractions indicated that these forms are made up by polypeptides of similar molecular weight (about 60-70 kDa). All CgA forms express the epitopes recognised by two monoclonal antibodies (A11 and B4E11), directed against residues 68-70 and 81-90 of human CgA. However, their relative immunoreactivity was markedly different. No evidence for the presence of multimeric complexes in the CgA-I fraction was obtained by various immunological and biochemical methods. These results suggest that circulating CgA in phaeochromocytoma patients consists of at least three forms that appear to be made up by polypeptides with similar molecular weight and different hydrodynamic properties an...Continue Reading

Citations

Sep 30, 2016·Oncotarget·Flavio CurnisAngelo Corti
Oct 20, 2018·Frontiers in Immunology·Elke M MuntjewerffGeert van den Bogaart
Nov 17, 2010·Cellular and Molecular Neurobiology·Angelo Corti
Mar 6, 2012·Journal of Molecular Neuroscience : MN·Y Peng LohBruno Tota
Sep 2, 2014·Frontiers in Chemistry·Bruno TotaMaria C Cerra
Jul 9, 2002·Clinical Endocrinology·Bernini GiampaoloSalvetti Antonio
Nov 28, 2009·Cellular and Molecular Life Sciences : CMLS·Bhavani S SahuNitish R Mahapatra
Feb 24, 2017·Frontiers in Endocrinology·Gautam K Bandyopadhyay, Sushil K Mahata
Nov 13, 2019·Journal of Hypertension·Saiful A MirSucheta M Vaingankar
Apr 25, 2000·Brain Research. Developmental Brain Research·N Cadet, J Paquin
Jun 15, 1997·Analytical Chemistry·A F HühmerC Schôneich

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