Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding of the rules governing complex formation and thereby functional flexibility

FEMS Microbiology Letters
Kirsty L LightbodyMark D Carr

Abstract

We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pairs of CFP-10/ESAT-6 family proteins (Rv0287/Rv0288 and Rv3019c/Rv3020c) and to determine whether complexes can be formed between non-genome paired members of the family. The results clearly demonstrate that Rv0287/Rv0288 and Rv3019c/3020c form tight complexes, as initially observed for CFP-10/ESAT-6. The closely related Rv0287/Rv0288 and Rv3019c/Rv3020c proteins are also able to form non-genome paired complexes (Rv0287/Rv3019c and Rv0288/Rv3020c), but are not capable of binding to the more distantly related CFP-10/ESAT-6 proteins.

Citations

Oct 22, 2010·Clinical and Vaccine Immunology : CVI·Leonardo Silva AraujoMaria Helena Féres Saad
Apr 17, 2007·Microbiological Research·Janka TeutschbeinThomas Munder
Sep 4, 2020·Microbiology and Molecular Biology Reviews : MMBR·Alexandra E ChirakosPatricia A Champion
Mar 22, 2016·Microbiology Spectrum·Louis S AtesWilbert Bitter

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