Characterisation of DDT, pyrethroid and carbamate resistance in Anopheles funestus from Obuasi, Ghana

Transactions of the Royal Society of Tropical Medicine and Hygiene
Patricia N OkoyeMaureen Coetzee

Abstract

Indoor-resting anopheline mosquitoes were collected from Obuasi, Ghana, and were identified morphologically and by PCR as Anopheles funestus Giles. Wild-caught females were induced to lay eggs. Samples of F1 progeny from each family were divided into cohorts and were either exposed to DDT and permethrin or were stored for biochemical analysis. Bioassay data by family show evidence of DDT and pyrethroid resistance in the parent A. funestus population. The sodium channel gene of DDT survivors and DDT-susceptible individuals was PCR amplified and sequenced to determine whether any kdr-type mutations were present. Molecular analysis of the IIS5-IIS6 segment of the sodium channel gene gave no indication of any kdr-type mutations associated with resistance phenotypes. Biochemical analysis suggests that DDT and pyrethroid resistance may be metabolically mediated, although there were no clear correlations between enzyme levels/activities and insecticide resistance across families. Furthermore, an altered acetylcholinesterase conferring carbamate resistance was evident. These results can be used to plan an effective malaria control strategy in the Obuasi region.

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Citations

Jan 16, 2013·Annual Review of Entomology·Maureen Coetzee, Lizette L Koekemoer
Aug 6, 2010·PloS One·John C MorganCharles S Wondji
Feb 27, 2013·The American Journal of Tropical Medicine and Hygiene·Michael R ReddyMichel A Slotman
Sep 17, 2009·Annual Review of Entomology·A Enayati, J Hemingway
Nov 6, 2012·Insect Biochemistry and Molecular Biology·Theresia Estomih NkyaJean-Philippe David
Apr 27, 2016·Parasites & Vectors·Marina Lidwine Olé SangbaMamadou Ousmane Ndiath
Feb 1, 2012·PloS One·Nicolas MoirouxVincent Corbel
Mar 19, 2014·The American Journal of Tropical Medicine and Hygiene·Dickson W LwetoijeraSilas Majambere
Jan 11, 2018·Parasites & Vectors·Leo BraackChristiaan de Jager

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