Characterisation of Dichelobacter nodosus and detection of Fusobacterium necrophorum and Treponema spp. in sheep with different clinical manifestations of footrot

Veterinary Microbiology
Sara FrosthAnna Aspán

Abstract

The aim of this study was to determine the proportion of Dichelobacter nodosus, Fusobacterium necrophorum and Treponema spp. in sheep with different clinical manifestations of footrot compared to healthy sheep both at flock and individual level. The second aim was to characterise D. nodosus with respect to virulence, presence of intA gene and the serogroups. Swab samples (n=1000) from footrot-affected (n=10) and healthy flocks (n=10) were analysed for the presence of D. nodosus, F. necrophorum and Treponema spp. by real-time PCR and culturing (D. nodosus only). Dichelobacter nodosus isolates (n=78) and positive swabs (n=474) were analysed by real-time PCR for the aprV2/B2 and the intA genes and by PCR for the fimA gene (isolates only). D. nodosus was more commonly found in flocks affected with footrot than in clinically healthy flocks. A significant association was found between feet with severe footrot lesions and the aprV2 gene and between feet with moderate or no lesions and the aprB2 gene, respectively. F. necrophorum was more commonly found in flocks with footrot lesions than in flocks without lesions. No significant association was found between sheep flocks affected with footrot and findings of Treponema spp. or the intA...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 1, 1968·Australian Veterinary Journal·N P Graham, J R Egerton
Mar 1, 1984·Australian Veterinary Journal·D L EmeryB L Clark
Apr 8, 2003·The Veterinary Record·G J WassinkL E Green
May 24, 2006·Veterinary Microbiology·B F CheethamM E Katz
Apr 4, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A JensenJ Prag
Oct 12, 2010·Veterinary Microbiology·G N Bennett, J G H Hickford
Apr 16, 2011·Acta Veterinaria Scandinavica·Ulrika KönigKerstin de Verdier
Jan 22, 2013·Veterinary Microbiology·Marianne GilhuusHannah J Jørgensen
Apr 17, 2014·Vaccine·Om DhungyelRichard Whittington
Jun 3, 2014·Acta Veterinaria Scandinavica·Marianne GilhuusHannah J Jørgensen

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Citations

Nov 10, 2015·Microbial Pathogenesis·Wenling GaoRory M Watt
Jun 19, 2016·The Veterinary Record·G MaboniS Tötemeyer
Sep 3, 2016·The Veterinary Record·Rachel Clifton, Laura Green
Nov 5, 2016·The Veterinary Journal·Synnøve Vatn
Feb 17, 2017·Journal of Clinical Microbiology·Andrew S McPhersonRichard J Whittington
Sep 30, 2017·Frontiers in Cellular and Infection Microbiology·Grazieli MaboniSabine Tötemeyer
Apr 10, 2018·Frontiers in Microbiology·Adam M BlanchardSabine Tötemeyer
Mar 25, 2017·Scientific Reports·Grazieli MaboniSabine Tötemeyer
Nov 7, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Deborah GreberAdrian Steiner
Apr 7, 2021·Journal of Wildlife Diseases·Gaia Moore-JonesMarie-Pierre Ryser-Degiorgis
Apr 13, 2021·The Veterinary Journal·Patrik ZanolariPeter Kuhnert
May 1, 2021·Animals : an Open Access Journal From MDPI·Julia StormsOttmar Distl
Aug 18, 2021·Acta Veterinaria Scandinavica·Malin BernhardUlrika König

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