PMID: 9166000Apr 1, 1997Paper

Characterisation of fibroblast-like cells in pannus lesions of patients with rheumatoid arthritis sharing properties of fibroblasts and chondrocytes

Annals of the Rheumatic Diseases
C XueK Nishioka

Abstract

To better understand the characteristics of synoviocytes located in the rheumatoid arthritis (RA) pannus. One cell line, termed PSC, was cloned from RA pannus lesions. Phenotypic analysis was done by contrast microscopy, indirect immunostaining, and safranin O staining. Transcription of several protooncogenes and matrix degrading enzymes was evaluated. The expression of mRNA for collagen II was detected by in situ hybridisation. The ability of anchorage independent growth was assessed by soft agarose culture. PSCs showed a high transcription of protooncogenes c-fos, c-myc and c-jun. They also expressed mRNA for matrix degrading enzymes, such as collagenase, cathepsin B, and cathepsin L. Anchorage independent growth assay demonstrated that PSCs formed colonies in soft agar culture. Phenotypic analysis showed that this fibroblast-like PSC was stained intensely with anti-vimentin and anti-fibroblast antibody. In situ reverse transcriptase assay showed that the cell line expressed type II collagen mRNA. Alternative fibroblast-like cells were identified in the pannus lesion of RA sharing properties of fibroblasts and chondrocytes. These findings suggest that this fibroblast-like cell derived from pannus lesions may contribute to the...Continue Reading

References

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Citations

Jan 1, 2000·Arthritis and Rheumatism·K NishimuraB Johnstone
May 23, 2008·Rheumatology International·Rim SghiriIbtissem Ghedira
May 25, 2005·Veterinary Immunology and Immunopathology·Narin UpragarinErik Gruys
Mar 21, 2012·Tissue Engineering. Part B, Reviews·Brendan A Jones, Ming Pei
Apr 3, 2004·Arthritis Research & Therapy·Hani S El-Gabalawy, John A Wilkins
Jul 25, 2006·Arthritis Research & Therapy·Christian DejacoManfred Herold
Nov 30, 2000·Arthritis Research·P P Tak, F C Breedveld
Oct 2, 2004·Clinical Rheumatology·Tomohiro KatoKusuki Nishioka
Dec 4, 1998·Springer Seminars in Immunopathology·T HasunumaK Nishioka
May 31, 2008·Clinical Orthopaedics and Related Research·Ming PeiGordana Vunjak-Novakovic
Mar 2, 2011·Clinical Orthopaedics and Related Research·Derek B Fox, Jennifer J Warnock

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