Characterisation of genes encoding key enzymes involved in sugar metabolism of apple fruit in controlled atmosphere storage

Food Chemistry
Zhu ZhuShiping Tian

Abstract

Sugars are essential contributors to fruit flavour. Controlled atmosphere (CA) storage has been proved to be beneficial for maintaining harvested fruit quality. To explore regulatory mechanism of sugar metabolism in fruit stored in CA condition, we cloned several genes, encoding key enzymes, involved in sugar metabolism in apple fruit, and analyzed sugar contents, along with gene expression and enzyme activities in fruits stored in air and CA. The results indicated that CA could maintain higher contents of sugars, including sucrose, fructose and glucose. Expression levels of key genes, such as sucrose synthase (SS), sucrose phosphate synthase (SPS), fructokinase (FK) and hexokinase (HK), were shown to be correlated with the corresponding enzyme activities. We found that activities of neutral invertase (NI), vacuolar invertase (VI), FK and HK were inhibited, but SPS activity was promoted in apple fruit stored in CA, suggesting that CA storage could enhance sucrose synthesis and delay hydrolysis of sucrose and hexose. These findings provided molecular evidence to explain why higher sugar levels in harvested fruit are maintained under CA storage.

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Citations

Jun 5, 2015·Food Chemistry·A L GalantJ D Wilson
Aug 25, 2018·Journal of the Science of Food and Agriculture·Yonghong GeXihong Li
Sep 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Di WangZisheng Luo
Jun 11, 2019·Frontiers in Plant Science·Jianghua CaiShiping Tian
Mar 7, 2020·Frontiers in Plant Science·Stefano BrizzolaraPietro Tonutti

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