PMID: 6411467Aug 15, 1983Paper

Characterisation of hepatocyte sub-populations generated by centrifugal elutriation

European Journal of Biochemistry
I G SumnerA Lodola

Abstract

We describe protocols for the fractionation of isolated hepatocytes into eight sub-populations using centrifugal elutriation. The distribution of fluorescein isothiocyanate and acridine orange in hepatocytes prepared from livers pre-perfused with one of these dyes is described and used as an indicator of acinar zone derivation for each population. The cytochrome P-450 content and response to induction by 3-methylcholanthrene and phenobarbitone; the distribution of lactate dehydrogenase, glucose-6-phosphatase, pyruvate kinase and tyrosine aminotransferase activities in the sub-populations is also reported. A marked asymmetry of distribution in all these activities was observed. On the basis of putative zone derivations (based on data of fluorescent dye distribution) of eight factors studied, the distributions of six were consistent with the sub-populations being derived from different acinar zones. Two major discrepancies were noted however, the distribution of pyruvate kinase activity and the response of the sub-populations to phenobarbitone. We conclude from this study that while a metabolic heterogeneity was revealed in the sub-populations generated, further characterisation is required to determine whether acinar zone separa...Continue Reading

References

Nov 15, 1977·FEBS Letters·F A Marston, C I Pogson
Mar 1, 1978·Chemico-biological Interactions·P E GoodingT F Slater
Mar 1, 1978·The American Journal of Physiology·J J GumucioM Keener
Oct 1, 1969·Experimental Cell Research·M Castagna, J Chauveau
May 1, 1982·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K Jungermann, N Katz
Jul 1, 1959·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A B NOVIKOFF

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Citations

Apr 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·B L KreamerH C Pitot
Jun 1, 1991·Toxicology Letters·B J KellerR G Thurman
Dec 29, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C TatenoK Yoshizato
Jan 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·I BraakmanG M Groothuis
Jul 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J C WiltonR Coleman
Sep 1, 1986·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J J GumucioV Massey
Oct 1, 1989·Cell Biochemistry and Function·K Rich, A Lodola

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