Characterisation of IncA/C2 plasmids carrying an In416-like integron with the blaVIM-19 gene from Klebsiella pneumoniae ST383 of Greek origin

International Journal of Antimicrobial Agents
C C PapagiannitsisJ Hrabák

Abstract

The complete nucleotide sequences of three multidrug resistance (MDR) IncA/C-like plasmids from Enterobacteriaceae isolates carrying the VIM-type carbapenemase-encoding integrons In4863 (blaVIM-19-aacA7-dfrA1-ΔaadA1-smr2) or In4873 (blaVIM-1-aacA7-dfrA1-ΔaadA1-smr2) were determined, which are the first In416-like elements identified in Greece. Plasmids pKP-Gr642 and pKP-Gr8143 were from Klebsiella pneumoniae ST383 isolates, whereas plasmid pEcl-Gr4873 was from an Enterobacter cloacae ST88 isolate. Sequencing showed that pKP-Gr642 (162787bp) and pKP-Gr8143 (154395bp) consisted of the type 1 IncA/C2 conserved backbone, the blaCMY-2-like gene-containing region, and the ARI-B (with the sul2 gene) and ARI-A (with a class 1 integron) resistance islands, like the plasmid pUMNK88_161 from the USA. The third plasmid, pEcl-Gr4873 (153958bp), exhibited extensive similarity with the type 2 IncA/C2 plasmid pR55 from France. pEcl-Gr4873 carried only one resistance island of a hybrid transposon structure inserted in a different location to ARI-A in type 1 A/C2 plasmids. In all three plasmids, the In416-like integrons In4863 or In4873 were identified within non-identical class II transposon structures. All three In416-like-carrying regions pre...Continue Reading

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Citations

Nov 2, 2017·The Journal of Antimicrobial Chemotherapy·Andreas E ZautnerWolfgang Bohne
Jun 2, 2016·Antimicrobial Agents and Chemotherapy·Nicole StoesserJohann D Pitout
Mar 19, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Tania Nawfal DagherJean-Marc Rolain
Jul 26, 2018·Frontiers in Microbiology·Veronika PaskovaJaroslav Hrabak
Apr 8, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Tom J B de ManBrandi M Limbago
Feb 10, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·May SherifAmani El-Kholy

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