Characterisation of membrane mimetics on a dual polarisation interferometer

Biosensors & Bioelectronics
Carla J TerryDavid G Fernig

Abstract

Dual polarisation interferometry (DPI) has been used to characterise the formation of hybrid bilayer membranes (HBM) on a silicon-oxynitride surface. This technique allows the simultaneous determination of multiple physical properties of an HBM, as the HBM is being formed in a single experiment: mass, thickness in the z-direction (normal to the surface), tilt angle of the first layer and refractive index. Decanoic acid was covalently attached to an amine modified silicon-oxynitride sensor chip surface via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride condensation reaction. The decanoic acid layer was 0.92+/-0.12 nm thick, indicating a tilt angle of 57 degrees from surface normal, and possessed a mass of 1.05+/-0.10 ng mm(-2) and a refractive index (RI) of 1.450+/-0.020. Phospholipid vesicles made from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) or 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) were injected over the fatty acid surface to form an HBM. The DPPC HBM was 4.32+/-0.68 nm thick, with a total mass of 3.18+/-0.60 ng mm(-2) and a RI of 1.404+/-0.007. The DMPC HBM was 2.12+/-0.34 nm thick, with a total mass of 2.25+/-0.51 ng mm(-2), and a RI of 1.435+/-0.007. DPI thus provides an insight into HBM...Continue Reading

References

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Citations

Mar 7, 2014·Biochimica Et Biophysica Acta·Tzong-Hsien LeeMarie-Isabel Aguilar
Mar 5, 2008·European Biophysics Journal : EBJ·Anton P Le BrunJeremy H Lakey
May 21, 2014·Analytical and Bioanalytical Chemistry·Daniel F KienleTonya L Kuhl
Feb 23, 2012·European Biophysics Journal : EBJ·David I FernandezFrances Separovic
Jan 27, 2010·Biochimica Et Biophysica Acta·Tzong-Hsien LeeMarie-Isabel Aguilar
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
May 20, 2006·Biochemical Society Transactions·E A YatesD G Fernig
Oct 8, 2009·The Journal of Physical Chemistry. B·Andreea OlaruEugen Gheorghiu
Mar 2, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Theodore J ZwangMalkiat S Johal
Apr 25, 2012·The Journal of Physical Chemistry. B·David Giménez-RomeroÁngel Maquieira
Dec 3, 2014·Chemical Reviews·Jorge EscorihuelaDavid Gimenez-Romero

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