Characterisation of mice lacking the inflammatory caspases-1/11/12 reveals no contribution of caspase-12 to cell death and sepsis

Cell Death and Differentiation
Ranja SalvamoserMarco J Herold

Abstract

Caspases exert critical functions in diverse cell death pathways, including apoptosis and pyroptosis, but some caspases also have roles in the processing of cytokines into their functional forms during inflammation. The roles of many caspases have been unravelled by the generation of knockout mice, but still very little is known about the overlapping functions of caspases as only a few studies report on double or triple caspase knockout mice. For example, the functions of caspase-12 in cell death and inflammation, on its own or overlapping with the functions of caspase-1 and caspase-11, are only poorly understood. Therefore, we generated a novel mutant mouse strain lacking all three inflammatory caspases, caspases-1, -11 and -12. Analysis under steady state conditions showed no obvious differences between caspase-1/11/12-/- and wildtype (WT) mice. Since caspases-1 and -11 are involved in endotoxic shock, we analysed the response of caspase-1/11/12-/- mice to high-dose LPS injection. Interestingly, we could not detect any differences in responses between caspase-1/11/12-/- mice vs. caspase-1/11 double knockout mice. Furthermore, cell lines generated from caspase-1/11/12-/- mice showed no differences in their apoptotic or necropt...Continue Reading

References

Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M J KosturaJ A Schmidt
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Aug 10, 2000·The American Journal of Pathology·L A O'ReillyA Strasser
Jul 13, 2001·The Journal of Biological Chemistry·R V RaoD E Bredesen
Jul 30, 2003·The Journal of Cell Biology·Michael KalaiPeter Vandenabeele
Dec 20, 2003·Cell Death and Differentiation·M LamkanfiP Vandenabeele
Dec 25, 2003·Diabetes·William H SchottEdward H Leiter
Jun 25, 2005·The Journal of Biological Chemistry·Esther A Obeng, Lawrence H Boise
Jan 13, 2006·Nature·Sanjeev MariathasanVishva M Dixit
Dec 22, 2007·Nature Reviews. Molecular Cell Biology·Richard J Youle, Andreas Strasser
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Feb 26, 2009·Immunity·Andreas StrasserShigekazu Nagata
May 29, 2009·The Journal of Biological Chemistry·Cristina Pop, Guy S Salvesen
Jul 25, 2009·Nature·Philipp J JostThomas Kaufmann
Dec 18, 2010·Trends in Molecular Medicine·Patrick J ShawThirumala-Devi Kanneganti
Feb 26, 2011·Nature Reviews. Immunology·Mohamed Lamkanfi
Aug 30, 2011·Medical Hypotheses·Evan Hermel, Kevin D Klapstein
Oct 18, 2011·Nature·Nobuhiko KayagakiVishva M Dixit
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
May 25, 2013·Nature Reviews. Immunology·Eicke LatzAndrea Stutz
Jul 28, 2013·Science·Nobuhiko KayagakiVishva M Dixit
Sep 10, 2013·Immunity·James M MurphyWarren S Alexander
Dec 3, 2013·Advances in Physiology Education·Rahul Suresh, David M Mosser
Dec 21, 2013·Nature Reviews. Molecular Cell Biology·Peter E CzabotarJerry M Adams
May 27, 2014·Cell·Mohamed Lamkanfi, Vishva M Dixit
Oct 18, 2014·Cold Spring Harbor Perspectives in Biology·Marcel R de ZoeteRichard A Flavell
Apr 17, 2015·Immunological Reviews·Si Ming Man, Thirumala-Devi Kanneganti
Oct 21, 2015·Cell Research·Si Ming Man, Thirumala-Devi Kanneganti
Jan 14, 2016·Arthritis Research & Therapy·Vitaliya SagulenkoJames E Vince
Apr 14, 2016·Cellular and Molecular Life Sciences : CMLS·James E Vince, John Silke
Feb 16, 2017·Clinical & Translational Immunology·Rebecca FelthamKate E Lawlor
Jan 14, 2018·Seminars in Cell & Developmental Biology·Monica L Gonzalez Ramirez, Guy S Salvesen

❮ Previous
Next ❯

Citations

Feb 6, 2020·Annual Review of Immunology·Sannula KesavardhanaThirumala-Devi Kanneganti
Sep 3, 2020·Nature Reviews. Molecular Cell Biology·Sammy BedouiAndreas Strasser
Nov 13, 2020·Frontiers in Cell and Developmental Biology·Barbora VeselaEva Matalova
Jul 14, 2021·Immunity·Michael Dominic StutzMarc Pellegrini

❮ Previous
Next ❯

Methods Mentioned

BETA
ICE
transfection
PCR
flow cytometry
ELISA
FCS
size fractioning
Protein Assay

Software Mentioned

Prism
Adobe Photoshop
FIJI
GraphPad Prism
GraphPad

Related Concepts

Related Feeds

Caspases in Metabolic Diseases

Caspases, the family of cysteine proteases are involved in programmed cell death, but their role in metabolic diseases, inflammation and immunity has been of interested. Discover the latest research on caspases in metabolic diseases here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Related Papers

Apoptosis : an International Journal on Programmed Cell Death
Selene García de la Cadena, Lourdes Massieu
Results and Problems in Cell Differentiation
L DorstynS Kumar
Biological Chemistry
Daniel Jiménez Fernández, Mohamed Lamkanfi
© 2022 Meta ULC. All rights reserved