PMID: 6645496Nov 1, 1983

Characterisation of monoclonal antibodies raised against testosterone

Journal of Steroid Biochemistry
V E Fantl, D Y Wang

Abstract

Using the antigens testosterone-17 beta-hemisuccinate and testosterone-3-(o-carboxymethyl) oxime, each coupled to bovine serum albumin, we have produced 44 monoclonal antibodies to testosterone. Of the 17 monoclonal antibodies raised against the 17 beta-linked antigen 8 showed extremely low affinity for testosterone (Ka less than or equal to 8 X 10(7) M-1) and none had an affinity greater than 5 X 10(9) M-1. Of the 27 monoclonal antibodies raised against the 3-linked antigen 2 had affinities less than 8 X 10(7) M, 7 had affinities greater than 5 X 10(9) M-1 and one had an affinity (Ka = 9 X 10(10) M-1) greater than that of a high affinity rabbit antiserum (Ka = 6 X 10(10) M-1). The affinity constant (Ka = 5 X 10(9) M-1) measured in the serum of the mouse whose spleen gave rise to the greatest number of high affinity antibodies, was significantly higher than those measured in the sera of the remaining mice (Ka = 0.7 - 3 X 10(8) M-1). The cross-reactions of the monoclonal antibodies varied widely but none showed an overall improvement in specificity when compared with the corresponding rabbit antisera. Results suggest that as well as the structure of the steroid antigen careful selection of the spleen donor facilitates the develo...Continue Reading

References

Nov 1, 1976·Clinical Endocrinology·D C AndersonA G Hendrickx
Apr 1, 1972·Journal of Steroid Biochemistry·W P CollinsI F Sommerville
Apr 1, 1981·Journal of Steroid Biochemistry·V E FantlA S Whitehead
Jan 1, 1980·Journal of Immunological Methods·S F de StGroth, D Scheidegger
Aug 1, 1982·Journal of Steroid Biochemistry·V E FantlR E Knyba

Citations

Feb 1, 1985·Journal of Steroid Biochemistry·A WhiteJ E Corrie
Jul 1, 1987·Journal of Steroid Biochemistry·M Fránek
Oct 1, 1987·Journal of Steroid Biochemistry·J SawadaT Nambara
Mar 1, 1986·Annals of Clinical Biochemistry·A A IsmailM Wheeler

Related Concepts

Monoclonal Antibodies
Immune complex
Cross Reactions
Hybridomas
Plasmacytoma
Sterotate

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