Characterisation of morphology of self-assembled PEG monolayers: a comparison of mixed and pure coatings optimised for biosensor applications

Analytical and Bioanalytical Chemistry
Jochen MehneGünter Gauglitz

Abstract

For detection of low concentrations of analytes in complex biological matrices using optical biosensors, a high surface loading with capture molecules and a low nonspecific binding of nonrelevant matrix molecules are essential. To tailor biosensor surfaces in such a manner, poly(ethylene glycols) (PEG) in varying lengths were immobilised covalently onto glass-type surfaces in different mixing ratios and concentrations, and were subsequently modified with three different kinds of receptors. The nonspecific binding of a model protein (ovalbumin, OVA) and the maximum loading of the respective analytes to these prepared surfaces were monitored using label-free and time-resolved reflectometric interference spectroscopy (RIfS). The three different analytes used varied in size: 150 kDa for the anti-atrazine antibody, 60 kDa for streptavidin and 5 kDa for the 15-bp oligonucleotide. We investigated if the mixing of PEG in different lengths could increase the surface loadings of analyte mimicking a three-dimensional matrix as was found using dextrans as sensor coatings. In addition, the effect on the surface loading was investigated with regard to the size of the analyte molecule using such mixed PEGs on the sensor surface. For further c...Continue Reading

References

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Citations

Apr 24, 2010·Analytical and Bioanalytical Chemistry·Marco FrasconiTommaso Ferri
Feb 18, 2011·Analytical and Bioanalytical Chemistry·Peter FechnerGünter Gauglitz
Mar 1, 2012·Analytical and Bioanalytical Chemistry·Meike V BeerJürgen Groll
Apr 6, 2013·Journal of the American Society for Mass Spectrometry·Ruixia ShiJin Ouyang
Nov 28, 2013·Analytical and Bioanalytical Chemistry·Oliver BleherGünther Proll
Feb 22, 2012·Analytical and Bioanalytical Chemistry·Urs HilbigGünter Gauglitz
Jul 12, 2014·Annual Review of Analytical Chemistry·Günter Gauglitz
Dec 10, 2015·Nanoscale·Lasse EvensenGareth Griffiths
Feb 12, 2019·Analytical and Bioanalytical Chemistry·Jonas BemetzMichael Seidel
Oct 12, 2017·Analytical and Bioanalytical Chemistry·Günter Gauglitz
Feb 18, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Michael SeifertHans-Joachim Galla
Aug 27, 2019·ACS Applied Materials & Interfaces·Lisa M MillerSteven D Johnson
Sep 11, 2021·Analytical and Bioanalytical Chemistry·Monika ConradGünter Gauglitz

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