PMID: 9545519Apr 18, 1998Paper

Characterisation of non-transferrin-bound iron (ferric citrate) uptake by rat hepatocytes in culture

Biochimica Et Biophysica Acta
E BakerE H Morgan

Abstract

Under conditions of iron overload plasma transferrin can be fully saturated and the plasma can transport non-transferrin-bound Fe which is rapidly cleared by the liver. Much of this Fe is complexed by citrate. The aim of the present work was to characterise the mechanisms by which Fe-citrate is taken up by hepatocytes using a rat hepatocyte cell culture model. The cells, after one day in culture, were incubated with 59Fe-labelled Fe-citrate for varying time periods, then washed and Fe uptake to the membrane and intracellular compartments of the cell was determined by radioactivity measurements. Maximal rates of internalisation of Fe occurred at a Fe:citrate molar ratio of 1:100 or greater, a pH of approximately 7.4 and an extracellular Ca2+ concentration of 1.0 mM. Fe uptake showed Michaelis-Menten kinetics and was a temperature-dependent process. The K(m) and Vmax for Fe internalisation by the cells at 37 degrees C were approximately 7 microM and 2 nmol/mg DNA/min (25 x 10(6) atoms/cell/min), respectively; and the Arrhenius activation energy was 35 kJ/mol. The transition metals, Zn2+, Co2+ and Ni2+, inhibited Fe uptake when used at 10 and 100 times the concentration of Fe. The rate of Fe internalisation from Fe-citrate was fou...Continue Reading

References

Oct 24, 1979·Biochimica Et Biophysica Acta·E H Morgan
Jul 15, 1976·European Journal of Biochemistry·H HuebersR R Crichton
Apr 9, 1991·Biochimica Et Biophysica Acta·Z M Qian, E H Morgan
Jun 7, 1991·Biochimica Et Biophysica Acta·D Richardson, E Baker
Sep 1, 1988·Biochimica Et Biophysica Acta·E H Morgan
Jul 1, 1986·The Biochemical Journal·E H MorganT J Peters
Nov 27, 1974·Biochimica Et Biophysica Acta·D Hemmaplardh, E H Morgan
Jan 1, 1971·Analytical Biochemistry·R T Hinegardner
Jul 1, 1993·The International Journal of Biochemistry·R A Løvstad
Dec 31, 1964·The New England Journal of Medicine·M S WHEBY, G UMPIERRE
Oct 30, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·J SuwinskiR Rittner
Aug 30, 2011·Environmental Science & Technology·Tijs M LammensImke J M De Boer
Apr 26, 2012·Molecular Biology and Evolution·J J BullC O Wilke

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Citations

Jan 24, 2002·Journal of Hepatology·Mark HirshTheodore C Iancu
Sep 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Juan P LiuzziRobert J Cousins
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·H E Smith-SomervilleH E Buhse
Aug 20, 2003·Molecular Biology of the Cell·Victoria A Kelley, Jeffrey S Schorey
Jul 16, 2002·Gut·D TrinderJ K Olynyk
Aug 15, 2000·Annual Review of Nutrition·M Wessling-Resnick
Feb 8, 2008·BMC Gastroenterology·Donald J Messner, Kris V Kowdley
May 15, 2015·Biomolecules·Nikolaus Bresgen, Peter M Eckl
Feb 13, 2016·EBioMedicine·Tomasa BarrientosNancy C Andrews
Aug 15, 2006·Blood Cells, Molecules & Diseases·Jan KovarJiri Horak
Aug 29, 2006·Journal of Theoretical Biology·Bert J Lao, Daniel T Kamei
Nov 8, 2005·Journal of Hepatology·Anita C G ChuaDebbie Trinder
Jun 16, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yan XuZhonghong Gao
Aug 23, 2011·Biochimica Et Biophysica Acta·Pierre BrissotOlivier Loréal
Jan 30, 2015·The Journal of Pharmacy and Pharmacology·Kanjana PangjitSomdet Srichairatanakool
Jun 16, 2016·Biochemistry. Biokhimii︠a︡·I V MiltoT K Klimenteva
Nov 24, 1999·The Journal of Laboratory and Clinical Medicine·E Becker, D R Richardson
Mar 19, 2008·The Journal of Biological Chemistry·Darius J R Lane, Alfons Lawen
Jul 25, 2000·The Journal of Biological Chemistry·J MwanjeweA K Grover
May 21, 2003·Critical Reviews in Clinical Laboratory Sciences·Jayong Chung, Marianne Wessling-Resnick
Oct 19, 2007·Critical Reviews in Clinical Laboratory Sciences·Anita C G ChuaJohn K Olynyk
Aug 4, 1998·The American Journal of Physiology·D Trinder, E Morgan
Jun 24, 2000·Endothelium : Journal of Endothelial Cell Research·R HallmannE Baker
Mar 16, 2013·Molecular and Cellular Biochemistry·Gérard LescoatFrançois Gaboriau
Sep 27, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·M E ConradM D Garrick
Jun 24, 2021·PloS One·Edouard Charlebois, Kostas Pantopoulos

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