Characterisation of P-glycoprotein-9.1 in Haemonchus contortus

Parasites & Vectors
Pablo GodoyRoger K Prichard

Abstract

The existence nematodes of veterinary importance such as Haemonchus contortus resistant to anthelmintic drugs, including the macrocyclic lactones, has become a major concern in animal health. Macrocyclic lactone resistance in H. contortus seems to be multigenic including the active efflux of these drugs by P-glycoproteins, members of the ABC transporter family, present in this parasite. The goals of the present work were to determine the activity of H. contortus P-glycoprotein 9.1 (Hco-PGP-9.1) and its interaction with the avermectins, ivermectin, abamectin, and also the milbemycin, moxidectin. Additionally, the localisation of Hco-PGP-9.1 was sought in adult worms. Hco-Pgp-9.1 was cloned and expressed in mammalian cells and its expression profile was determined at the transcriptional and protein level by qRT-PCR and Western-blot, respectively. The nematode transport activity was assessed using the tracer dye Rhodamine 123. A ligand competition assay between different macrocyclic lactones and Rhodamine 123 was used to establish whether or not there was interaction between Hco-PGP-9.1 and the avermectins (abamectin and ivermectin) or moxidectin. In addition, immunostaining was carried out to localise Hco-PGP-9.1 expression in th...Continue Reading

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Citations

May 14, 2016·International Journal for Parasitology·Thangadurai ManiRoger K Prichard
Oct 18, 2016·International Journal for Parasitology. Drugs and Drug Resistance·Marion A DavidAnne Lespine
Jun 2, 2016·Antimicrobial Agents and Chemotherapy·Cécile MénezAnne Lespine
Aug 14, 2020·Scientific Reports·Alexander P GerhardGeorg von Samson-Himmelstjerna
Jul 11, 2018·Antimicrobial Agents and Chemotherapy·Ivona MladineoConcetta Messina
Jun 24, 2019·International Journal for Parasitology. Drugs and Drug Resistance·Roger K Prichard, Timothy G Geary

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Methods Mentioned

BETA
transgenic
protein assay
PCR
transfection
reverse transcription-PCR
ELISA
dot blot

Software Mentioned

MOX
Geneious
ImageJ
GraphPad Prism

Related Concepts

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Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.