Characterisation of phenolic compounds in South African plum fruits (Prunus salicina Lindl.) using HPLC coupled with diode-array, fluorescence, mass spectrometry and on-line antioxidant detection

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Alet VenterDalene de Beer

Abstract

Phenolic compounds are abundant secondary metabolites in plums, with potential health benefits believed to be due to their antioxidant activity, amongst others. Phenolic characterisation of South African Prunus salicina Lindl. plums is necessary to fully evaluate their potential health benefits. An HPLC method using diode-array detection (DAD) for quantification of phenolic compounds was improved and fluorescence detection (FLD) was added for quantification of flavan-3-ols. Validation of the HPLC-DAD-FLD method showed its suitability for quantification of 18 phenolic compounds, including flavan-3-ols using FLD, and phenolic acids, anthocyanins and flavonols using DAD. The method was suitable for characterisation of the phenolic composition of 11 South African plum cultivars and selections, including various types with yellow and red skin and flesh. The method was used in conjunction with mass spectrometry (MS) to identify 24 phenolic compounds. Neochlorogenic acid and cyanidin-3-O-glucoside were the major compounds in most of the plums, while cyanidin-3-O-glucoside was absent in Sun Breeze plums with yellow skin and flesh. Post-column on-line coupling of the ABTS•+ scavenging assay with HPLC-DAD enabled qualitative evaluation o...Continue Reading

References

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Citations

Mar 13, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alet VenterDalene de Beer
Feb 26, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Christiaan J MalherbeElizabeth Joubert
Jul 5, 2017·Journal of the Science of Food and Agriculture·Emilie StierlinThomas Michel
Feb 2, 2019·International Journal of Food Sciences and Nutrition·Valentina PicchiWilfried Schwab
Sep 29, 2020·Frontiers in Plant Science·María Valeria LaraMaría Fabiana Drincovich
May 17, 2021·Food Research International·Jiawen YuRuili Yang
Dec 17, 2014·Journal of Agricultural and Food Chemistry·Valentina UsenikMojca Virscek Marn

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Methods Mentioned

BETA
Fluorescence

Software Mentioned

MassLynx
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