Characterisation of porcine epidemic diarrhea virus isolates during the 2014-2015 outbreak in the Philippines

Virusdisease
Gemerlyn G GarciaClaro N Mingala

Abstract

The viral agent of the porcine epidemic diarrhea (PED) was investigated during the reported 2014-2015 outbreaks in commercial farms in Central Luzon, Philippines. The study covered detection of PED virus (PEDV) in fecal and intestinal samples through reverse transcription PCR and sequence analysis of the nucleocapsid (N) gene. Results showed that 10 out of 34 fecal and intestinal samples examined were positive for PEDV. The partial nucleotide sequence of the N gene of the field samples showed 98-99% homologous to PEDV sequences registered in the GenBank. It was also noted that N gene sequences between field samples were 98% homologous. Interestingly, the partial sequences of the N genes of the field samples were genetically similar to the PEDV isolates from USA, China, Mexico, Canada and Japan. The phylogenetic tree analysis revealed that the Philippine samples clustered in group 2-1 of the PEDV, wherein the isolates of this group were responsible for the outbreaks in Asia and the USA. Analysis of the partial nucleotide and amino acid sequences revealed polymorphisms, deletions and insertions in the N-gene of the PEDV. Amino acid sequence alignment also showed deletions and insertion in the PEDV detected in the Philippines.

References

Jan 1, 1978·Archives of Virology·M B Pensaert, P de Bouck
Mar 19, 1977·The Veterinary Record·E N Wood
Jan 21, 2012·Emerging Infectious Diseases·Rui-Qin SunChang-Xu Song
Apr 23, 2014·Emerging Infectious Diseases·Leyi WangYan Zhang
Jun 6, 2014·The Journal of Veterinary Medical Science·Chao-Nan LinMing-Tang Chiou
Jun 25, 2014·Emerging Infectious Diseases·Sunhee Lee, Changhee Lee
Aug 16, 2014·Genome Announcements·Dam Thi VuiDachrit Nilubol
Oct 4, 2014·Emerging Infectious Diseases·Anastasia N VlasovaLinda J Saif
Nov 25, 2014·Emerging Infectious Diseases·Douglas MarthalerKurt Rossow
Aug 15, 2015·Genome Announcements·Satoshi MurakamiTohru Suzuki
Apr 9, 2016·Genome Announcements·Ivan ToplakNataša Toplak
Apr 21, 2016·Polish Journal of Veterinary Sciences·Ren-Feng LiDong-Yang Liu
May 24, 2016·Virus Research·Samaporn TeeravechyanAnan Jongkaewwattana
Jun 29, 2016·Archives of Virology·Rubigilda Paraguison-Alili, Clarissa Yvonne J Domingo

❮ Previous
Next ❯

Citations

Sep 26, 2020·Veterinary Medicine and Science·Médiha Khamassi KhbouM'hammed Benzarti

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

Basic Local Alignment Search Tool ( BLAST )
MEGA
MUSCLE

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.