Characterisation of proteins in excretory/secretory products collected from salmon lice, Lepeophtheirus salmonis

Parasites & Vectors
Scott HamiltonDavid Knox

Abstract

The salmon louse, Lepeophtheirus salmonis, is an ectoparasitic copepod which feeds on the mucus, skin and blood of salmonid fish species. The parasite can persist on the surface of the fish without any effective control being exerted by the host immune system. Other ectoparasitic invertebrates produce compounds in their saliva, excretions and/or secretions which modulate the host immune responses allowing them to remain on or in the host during development. Similarly, compounds are produced in secretions of L. salmonis which are thought to be responsible for immunomodulation of the host responses as well as other aspects of crucial host-parasite interactions. In this study we have identified and characterised the proteins in the excretory/secretory (E/S) products of L. salmonis using LC-ESI-MS/MS. In total 187 individual proteins were identified in the E/S collected from adult lice and pre-adult sea lice. Fifty-three proteins, including 13 serine-type endopeptidases, 1 peroxidase and 5 vitellogenin-like proteins were common to both adult and pre-adult E/S products. One hundred and seven proteins were identified in the adult E/S but not in the pre-adult E/S and these included serine and cysteine-type endopeptidases, vitellogenin...Continue Reading

References

Jul 1, 1990·Clinical and Experimental Immunology·S S To, L Schrieber
May 1, 1982·British Journal of Pharmacology·J Haylor, J Towers
Jan 1, 1995·Annual Review of Entomology·J R SauerR C Essenberg
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·A ShevchenkoM Mann
Nov 24, 1999·Advances in Parasitology·A W Pike, S L Wadsworth
Feb 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Natalia FriedlandAnn M Stock
Oct 28, 2005·Rapid Communications in Mass Spectrometry : RCM·Gregory K Taylor, David R Goodlett
Jun 15, 2006·Rapid Communications in Mass Spectrometry : RCM·Mariola BatyckaBenedikt M Kessler
May 1, 2008·Nucleic Acids Research·Stefan GötzAna Conesa
Apr 1, 2009·Infection and Immunity·Xiuyang GuoRuth R Montgomery
Jul 15, 2009·Insect Biochemistry and Molecular Biology·Margaret WerrThomas Ilg
May 27, 2010·Parasite Immunology·M W RobinsonS Donnelly
Jul 13, 2011·Marine Biotechnology·Motoshige YasuikeBen F Koop
Aug 9, 2011·International Journal for Parasitology·Zdenek FrantaPetr Kopacek
Sep 14, 2013·Ticks and Tick-borne Diseases·C J OliveiraJ M C Ribeiro
Jul 13, 2016·Immunological Investigations·Samanta S Khora

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Methods Mentioned

BETA
RNAseq
protein assay
electrophoresis

Software Mentioned

Dionex
Blast2Go
Matrix Science
Proteinscape
[UNK] chromatography
Mascot
[UNK]
SignalP

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